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高通量检测在水厂处理工艺中的应用
引用本文:吴伟健,孔义军,马薇,赵倩宁.高通量检测在水厂处理工艺中的应用[J].中国卫生检验杂志,2020(4):399-401.
作者姓名:吴伟健  孔义军  马薇  赵倩宁
作者单位:澳门自来水股份有限公司
摘    要:目的探讨高通量检测在水厂处理工艺中的应用。方法本文尝试利用高通量分析从源头水(西江末端磨刀门地表水),各个水处理工艺出水,出厂处理水以及城市内的管网水,希望利用实验结果,分析从源头至用户端水体内微生物种类、族群组成及变化情况。结果分析相似度97%OTU种类结果显示,水厂具有良好的微生物去除效果,以前加氯结合混凝效果最为显著,最高去除物种数目可达到91. 03%,符合预期。另一方面,水厂内部由于长期运行,过往无法去除的微生物可能由内部运行后产生二次污染。结论最后检测每个样品的DNA序列结果,经过水厂整个处理工序后,出厂水没有找到具高危性的致病菌,可以确保饮用水的安全性。

关 键 词:高通量  操作分类单元  DNA序列检测  致病菌

Application of high-throughput technology in water treatment process
WU Wei-jian,KONG Yi-jun,MA Wei,ZHAO Qian-ning.Application of high-throughput technology in water treatment process[J].Chinses Journal of Health Laboratory Technology,2020(4):399-401.
Authors:WU Wei-jian  KONG Yi-jun  MA Wei  ZHAO Qian-ning
Institution:(The Macao Water Supply Company Limited,Macao 999078,China)
Abstract:Objective To discuss the application of high-throughput technology in water treatment process from our local water treatment plants. Methods This article attempts to use high-throughput analysis and experimental results to analyze the microbial species,ethnic composition,and changes in the water body from source to user end,and the samples were respectively collected from the source water( surface water at Modaomen at the end of the Xijiang River),various water treatment processes,the factory treated water,and even the pipe network water in the city. Results Similarity analysis of 97% OTU species shows that the water plant has a good microbial removal effect. The previous chlorination and coagulation effect was the most significant,and the maximum number of removed species could reach 91. 03%,which was in line with expectations. On the other hand,due to the long-term operation,microorganisms that could not be removed in the past may cause secondary pollution after internal operation. Conclusion After water treatment process,there were no pathogens at high risks found in all our treated water based on the DNA sequencing test results,and the provable safety for our drinking water can be ensured.
Keywords:High-throughput  OTU  DNA sequencing test  Pathogens
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