| 血清预培养促进神经干细胞的增殖 |
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| 引用本文： | 历俊华,郑淑荣,万虹,张绍东.血清预培养促进神经干细胞的增殖[J].中国康复理论与实践,2005,11(5):339-340. |
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| 作者姓名： | 历俊华 郑淑荣 万虹 张绍东 |
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| 作者单位： | 100050,北京市,北京市神经外科研究所 |
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| 基金项目： | 国家自然科学基金(No.30370543) |
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| 摘 要： | 目的 介绍一种时间短、花费少的神经干细胞培养方法。方法 先用含10％胎牛血清的DMEM培养液预培养神经干细胞48h，换含表皮生长因子、碱性成纤维细胞生长因子、B27的DMEM／F12培养液继续培养干细胞球。血清诱导分化后行nestin免疫荧光染色。结果 血清预培养48h后即可见神经干细胞聚球，nestin免疫荧光染色为阳性。结论 血清预培养可使神经干细胞聚球速度加快，促进神经干细胞增殖。
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| 关 键 词： | 神经干细胞 预培养 血清 增殖 |
| 文章编号： | 1006-9771(2005)05-0339-02 |
| 修稿时间： | 2005年1月31日 |
Pro-culture with serum promoting neural stem cell proliferation |
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| LI Jun-hua,ZHENG Shu-rong,WAN Hong,et al..Pro-culture with serum promoting neural stem cell proliferation[J].Chinese Journal of Rehabilitation Theory and Practice,2005,11(5):339-340. |
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| Authors: | LI Jun-hua ZHENG Shu-rong WAN Hong |
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| Institution: | LI Jun-hua,ZHENG Shu-rong,WAN Hong,et al. Beijing Neurosurgical Institute,Beijing 100050,China |
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| Abstract: | ObjectiveTo introduce a method of promoting neural stem cells proliferation.MethodsNeural stem cells were cultured with medium DMEM containing 10% fatal bovine serum for 48 h, then changed medium DMEM/F12 containing epidermal growth factor, basic fibroblast growth factor and B27. Immunohistochemistry was used to identify nestin expression.ResultsNeural stem cells got together fast and formed neurospheres within 48 h and expressed nestin. ConclusionPro-culture with serum can promotes neural stem cell proliferation. |
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| Keywords: | neural stem cell pre-culture serum proliferation |
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