抗衰片对电离辐射损伤后小鼠骨髓造血作用的影响

摘要： 目的 探讨抗衰片对电离辐射损伤后小鼠造血重建的调控影响。方法 9~10 周龄雄性 C57BL/6 小鼠随机分为照射对照组、 低剂量组、 中剂量组和高剂量组， 每组 10 只。后 3 个剂量组分别按 0.75、 1.5、 3.0 g/kg 体质量灌服抗衰片水溶液， 照射对照组给予同体积生理盐水， 1 次/d， 连续给药 11 d。第 4 天对 4 组小鼠进行 6.0 Gy 137 Cs-γ射线单次全身照射。照射后第 8 天， 观察小鼠内源性脾结节 （即脾集落形成单位， CFU-S）、 小鼠骨髓粒-巨噬细胞集落形成单位 （CFU-GM） 和骨髓成纤维细胞集落形成单位 （CFU-F） 的生成情况。同时， 体外扩增的骨髓间充质干细胞（MSC） 与正常供体小鼠的造血干细胞 （HSC） 进行二维共培养 （2D CFU-GM）， 通过 2D CFU-GM 检测辐射损伤后药物作用的 MSC 促进 HSC 的增殖能力。结果 与照射对照组相比， 3 个剂量组的 CFU-GM 数目显著增多， 中剂量组及高剂量组 CFU-S、 CFU-F、 2D CFU-GM 增殖能力显著提高 （均 P＜0.05）。结论 抗衰片可以促进电离辐射损伤后小鼠造血系统重建。

The effects of KS tablet on hematopoietic regulation in mice treated with radiation

Abstract： Objective To investigate the effects of Kangshuai (KS) tablet on the hematopoietic reconstitution in mice treated with radiation. Methods C57BL/6 mice were randomly divided into four groups: irradiated control group, low-dosegroup (0.75 g/kg), middle-dose group (1.5 g/kg) and high-dose group (3.0 g/kg). There were ten mice for each group. The mice in three treated groups were given KS tablet suspension orally for three days before the treatment with irradiation, and the control group was given equal quantity of normal saline. All the mice were underwent irradiation of 6.0 Gy 137 Cs-γ rays, and were continuously given drug suspension or saline for seven days. The mice were sacrificed on the eighth day after irradiation. Colony forming unit of spleen (CSF-S), granulocyte macrophage colony-forming unit (CFU-GM) and fibroblast colony-form⁃ ing unit (CFU-F). The 2D CFU-GM hematopoietic stem cells (HSC) were co-cultured with mesenchymal stem cell (MSC). Re⁃ sults Compared with the irradiated control group, numbers of CFU- GM were significantly increased in three treatment groups. The proliferations of CFU-S, CFU-F and 2D CFU-GM were significantly enhanced in middle and high dose groups (P＜0.05). Conclusion KS tablet can promote hematopoietic reconstitution in mice after irradiation damage.