PCNA和COX-2在喉鳞状细胞癌中的表达及意义

目的探讨增殖细胞核抗原（PCNA）和环氧化酶-2（COX-2）在喉鳞状细胞癌组织中的表达及其与肿瘤发生和增殖的关系。方法用免疫组化SP法检测65例喉鳞癌组织及34例癌旁非肿瘤组织中PCNA和COX-2的表达，并分析两者的表达水平与各临床病理参数（年龄、吸烟、病程、饮酒、TN分期、病理分级）的关系。结果PCNA和COX-2在肿瘤组织中的阳性表达率分别为66．2％和72．3％，与对照癌旁非肿瘤组织比较（29．4％，20．6％）其表达显著增高，差别均有统计学意义。且PCNA和COX-2的表达相关。Logistic回归分析结果，t分期愈高、病程愈长易出现COX-2表达，淋巴结转移易出现PCNA表达。结论喉鳞癌组织中PCNA和COX-2的过度表达可能促进了喉鳞癌的发生发展，且两者在肿瘤的发生和发展中可能存在协同作用，PCNA和COX-2是喉鳞癌可能的治疗靶点。

The expression and significance of PCNA and COX-2 in laryngeal squamous cell carcinoma

Objective To investigate the expression of PCNA and COX-2 in laryngeal squamous cell carcinoma （LSCC） and the role in the tumorigenesis and proliferation of LSCC. Methods The expression of PCNA and COX-2 in 65 primary LSCC tissues and 34 adjacent non-neoplastic tissues were detected with immunohistochemical SP method. The relationship of PCNA and COX-2 and age, smoking, duration, alcohol consuming, TN staging, differentiation and clinical typing were studied. Results The expression of PCNA and COX-2 was observed in 66.2% and 72.3% of the case at the primary site and in 29.40% and 20.6 % of the adjacent non-plastic tissues, which demonstrated a significant difference from each other （P〈0.01）. In addition,there was a significant correlation between the expression of PCNA and COX-2 （P〈0.01）. The expression of PCNA and COX-2 in T3-4 stages LSCC was obviously higher than those in T1-2 stages（p 〈0. 05）. The expression of PCNA in the Lymphaden transfered group was higher than that of the untransfered group（P 〈0.05）, but the expression of COX-2 was not. There were no significant correlation between the expression of PCNA and cox-2 with other variables, including age, duration, smoking, alcohol consuming, differentiation and clinical typing. Conclusion The overexpression of COX-2 and PCNA was observed in LSCC tissues, which might play a crucial role in oneogenesis and proliferation of LSCC.