龙葵碱体外诱导人结肠癌SW480细胞凋亡及其对相关因子的影响

  目的：观察龙葵碱对人结肠癌SW480细胞凋亡及ERK/MEK、PI3K/AKT信号通路中相关因子的影响。  方法：SW480结肠癌细胞培养后共设5组,分别为空白对照组、培养液对照组及龙葵碱不同剂量(4、8、16 mmol/L)组,各组进行相应干预。采用MTT法检测龙葵碱对结肠癌细胞株的增殖作用,流式细胞法检测龙葵碱对结肠癌细胞凋亡的影响,RT-PCR及Western blotting法检测龙葵碱处理的SW480细胞中ERK/MEK和PI3K/AKT的表达水平。  结果：MTT生长曲线显示,龙葵碱能抑制结肠癌SW480细胞株增殖。流式细胞仪检测显示,龙葵碱能够促进SW480的凋亡。RT-PCR结果显示,龙葵碱可以抑制ERK1/2 mRNA的表达。Western blotting结果表明,龙葵碱可以降低MEK/ERK和PI3K/AKT信号通路中相关蛋白的表达。  结论：龙葵碱可以抑制结肠癌细胞增殖,其对肿瘤的诱导凋亡作用可能是通过MEK/ERK和PI3K/AKT信号途径实现的。

Effects of solanine in vitro induction SW480 cell apoptosis and related factors of human with colon cancer

  Objective:To observe the effects of solanine on SW480 cell apoptosis of human with colon cancer and the related factors in ERK/MEK, PI3K/AKT signal pathway. MethodsThe SW480 colon cancer cell was divided into 5 groups after the cultivation, blank control group, culture solution control group and solanine 4,8, 16 mmol/L groups, each group administered with intervention respectively. The proliferation function of solanine on colon cancer cell line was tested with MTT method, the effects of solanine on colon cancer cell apoptosis was tested with FCM, the expression of ERK/MEKand PI3K/AKT in SW480 cell that treated with solanine was detected with RT-PCR and Western blotting method.  Results:MTT growth curve showed that solanine could inhabite the proliferation of colon cancer SW480 cell line. Flow Cytometry showed that solanine could promote the SW480 cell apoptosis. RT-PCR showed that solanine could inhabite the expression of ERK1/2 mRNA. Western blotting showed that solanine could decrease the protein expression in the MEK/ERK and PI3K/AKT signal pathway.  Conclusion:Solanine could inhabite the proliferation of colon cancer, and the cell apoptosis induction was realized may be though MEK/ERK and PI3K/AKT signal pathway.