| 抗乳腺癌人鼠嵌合抗体的构建 |
| |
| 引用本文： | 樊晓晖,杨海波. 抗乳腺癌人鼠嵌合抗体的构建[J]. 广西医科大学学报, 2002, 19(1): 19-21 |
| |
| 作者姓名： | 樊晓晖 杨海波 |
| |
| 作者单位： | 广西医科大学微生物及免疫学教研室,南宁,530021;广西医科大学微生物及免疫学教研室,南宁,530021 |
| |
| 基金项目： | 广西教育厅留学人员回国项目资助 |
| |
| 摘 要： | 目的：构建抗人乳腺癌单克隆抗体人鼠嵌合抗体,降低鼠源性抗体应用于人体的免疫排斥反应。方法：采用RT－PCR技术,从分泌抗人乳腺癌单克隆抗体的杂交瘤细胞株CDI315B分离克隆抗体轻重链可变区基因,对其进行序列分析,将连接组建人鼠嵌合轻链和人鼠嵌合重链,然后分别将它们克隆到真核细胞表达载体pcDNA3。结果：构建的人鼠嵌合抗体基因克隆在真核细胞表达载体上。结论：人鼠嵌合抗体的构建为它的表达和临床应用打下基础。
|
| 关 键 词： | 乳腺癌 嵌合抗体 克隆 |
| 修稿时间： | 2001-03-27 |
CONSTRUCTION OF HUMAN-MOUSE CHIMERIC ANTIBODY AGAINST BREAST CANCER |
| |
| Fan Xiaohui,Yang Haibo. CONSTRUCTION OF HUMAN-MOUSE CHIMERIC ANTIBODY AGAINST BREAST CANCER[J]. Journal of Guangxi Medical University, 2002, 19(1): 19-21 |
| |
| Authors: | Fan Xiaohui Yang Haibo |
| |
| Abstract: | Objective: To construct an anti breast cancer human mouse chimeric antibody and reduce its human anti mouse antibody response in vivo application Methods: The entire VH and VL genes of anti breast cancer McAb CDI 315B were amplified by RT PCR Mouse VH and VL genes were joined to human Cr1 gene and human CL gene and the recombination genes were subsequently subcloned into expression vector pcDNA3 Results: Chimeric antibody was constructed in an eukaryotic expression vector pcDNA3 Conclusion: This chimeric antibody will be further expressed in mammalian cells and provide a new approach to utilize human mouse chimeric antibody in clinical treatment of patients with breast cancer |
| |
| Keywords: | breast cancer chimeric antibody clone |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
