喘敷灵穴位贴敷对哮喘模型大鼠疗效机制的探讨

目的:探讨喘敷灵巴布剂防治哮喘的作用机制.方法:将30只雌性SD犬鼠随机分为正常组(生理盐水腹腔注射+空白敷贴+生理盐水雾化)、模型组(卵蛋白生理盐水腹腔注射+空白敷贴+卵蛋白生理盐水雾化)、敷贴组(卵蛋白生理盐水腹腔注射+喘敷灵巴布剂敷贴+卵蛋白生理盐水雾化),每组10只.观察各组大鼠发生点头呼吸的潜伏期、哮喘发作症状;检测各组大鼠白介素4信使RNA(IL-4 mRNA),γ-干扰素信使RNA(γ-IFN mRNA)的表达;观察右肺中叶肺组织病理变化.结果:①与正常组比较,模型组外周血单核细胞(PBMC)中IL-4 mRNA表达增强、肺组织充血渗出及嗜酸性粒细胞(EOS)浸润严重(P＜0.01);②与模型组比较,敷贴组PBMC中IL-4 mRNA降低,第5、7次诱喘发生点头呼吸的潜伏期延长、频率减少、肺组织EOS浸润减轻(P＜0.05),而肺组织充血渗出无显著差异(P＞0.05).结论:喘敷灵巴布剂能抑制哮喘大鼠PBMC中IL-4基因的转录及气道内EOS释放炎症介质、细胞因子,减轻气道上皮损伤和气道高反应性.

Study on the mechanism of acupoint sticking therapy with Chuanfuling for treatment of asthma model rats

Objective To investigate the mechanism of the acupoint sticking therapy with Chuanfuling for preventing and treating asthma. Methods Thirty male SD rats were randomly divided into a control group (normal saline, p. i.+no acupoint sticking+normal saline, spray inhalation) , model group (normal saline with ovalbumin, p. i. +no acupoint sticking+normal saline with ovalbumin, spray inhalation), and acupoint sticking group (normal saline with ovalbumin, p. i. + acupoint sticking with Chuanfuling+normal saline with ovalbumin, spray inhalation),10 rats in each group. The incubation period of nodding breath, symptom of asthmatic attack, expression level of interleukin-4 mRNA (IL-4 mRNA) and interferon-y mRNA (IF-y mRNA), as well as pathological changes on the middle leaf of right lung, were observed in each group. Results (1) Comparing with the control group, the model group was showed that the expression level of IL-4 mRNA in the peripheral blood cells (PBMC) was increased,while hyperemia, edema and eosinocyte (EOS) invasion of lung tissue was more serious (P<0. 01). (2) Comparing with the model group, the acupoint sticking group was showed that the expression level of IL-4 mRNA in PBMC was decreased, the incubation period of nodding breath was prolonged for induced asthma on the fifth and seventh time with lower frequency, while in the lung tissue EOS invasion was reduced (P<0. 05) , but there were no significant changes on the hyperemia and edema (P>0. 05). Conclusion Acupoint sticking for treating asthma of model rats with Chuanfuling can inhibit the expression level of IL-4 mRNA in PBMC, and the release of the inflammatory mediator and cytokine from the EOS to the air passage, in order to reduce the injury of epithelial layer and high reaction on the air passage.