Ultrathin-layer starch gel electrophoresis--a method qualified to solve specific problems in enzyme analysis |
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Authors: | H G D?ssler T Schmiedel |
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Affiliation: | Sektion Forstwirtschaft, Technischen Universit?t Dresden. |
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Abstract: | Particular separation problems of enzymatic analysis led to optimization investigation on starch gel electrophoresis. Investigations on the optimum gel concentration range a well as on the duration of the hydrolysis of starch for a suitable gel network gave rise to a starch level with good molecular sieve effect. Optimization of the electrophoretic buffer system and the development of an optimum technique for manufacturing handy transparent ultra thin-layer starch gels with high selectivity results for a broad spectrum of diagnostically enzymatic systems has turned out to be of advantage for enzymatic electrophoresis. |
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