| 高效液相色谱法测定氟康唑注射液的含量及有关物质 |
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| 引用本文： | 王娅娟,逄书明,石艳萍.高效液相色谱法测定氟康唑注射液的含量及有关物质[J].中国医院药学杂志,2003,23(5):268-270. |
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| 作者姓名： | 王娅娟 逄书明 石艳萍 |
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| 作者单位： | 1. 山东教育学院化学系,山东,济南,250013
2. 山东省医药工业研究所,山东,济南,250100 |
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| 摘 要： | 目的:建立用高效波相色谱法(HPLC)法测定氟康唑注射液的含量及有关物质的方法。方法:用十八烷基硅烷键合硅胶为填充剂,pH7.0的磷酸盐缓冲液-甲醇(55:45)为流动相,检测波长为260nm。结果:氟康唑注射液在浓度60～140mg·L~(-1)范围内,溶液的浓度与峰面积呈良好的线性关系。精密度试验RSD为0.35％(n=5),最小检出量为10ng,检出限度为0.05％。氟康唑注射液含量测定的平均回收率为99.7％,RSD为0.53％(r=5)。结论:本方法简单,快速,准确,可用于氟康唑注射液的含量测定和有关物质的检查。
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| 关 键 词： | 高效液相色谱法 测定 氟康唑注射液 含量 |
| 文章编号： | 1001-5213(2003)05-0268-03 |
| 修稿时间： | 2002年7月13日 |
Dtermination of fluconazole injection and its related substance by HPLC |
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| WANG Ya-Juan,PANG Shu-Ming,SHI Yan-Ping.Dtermination of fluconazole injection and its related substance by HPLC[J].Chinese Journal of Hospital Pharmacy,2003,23(5):268-270. |
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| Authors: | WANG Ya-Juan PANG Shu-Ming SHI Yan-Ping |
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| Abstract: | OBJECTIVE To establish a method for determining the content and related substance of fluconazole injection by HPLC. METHODS The C18 column was used and the mobile phase was consisted of pH 7.0 phosphate-methanol(55:45). The detection wavelength was at 260 nm. RESULTS A good linearity was obtained in the concentration range of 60~ 140 mg.L-1 ,and the average recovery of fluconazole was 99.7% , RSD = 0.53%(n=5). CONCLUSIONS This method is simple, rapid, accurate and can be used to determine fluconazole and its related substance. |
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| Keywords: | HPLC fluconazole related substance injection contents determining |
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