骨髓间充质干细胞移植大鼠脑缺血区微血管密度和肝细胞生长因子的表达

背景：应用骨髓间充质干细胞移植治疗脑缺血可促进损伤神经功能的恢复,目前其作用机制尚未明确。目的：分析骨髓间充质干细胞移植对大鼠脑缺血保护作用的机制。方法：采用线栓法复制大鼠大脑中动脉栓塞模型,随机分为假手术组、大脑中动脉栓塞组、溶剂对照组和骨髓间充质干细胞组。骨髓间充质干细胞组于脑梗死1d后经侧脑室注射入骨髓间充质干细胞,溶剂对照组则注射同等剂量的PBS。结果与结论：大鼠脑缺血后缺血区皮质可见大量的微血管生成,2周达高峰。骨髓间充质干细胞组缺血区微血管密度显著高于大脑中动脉栓塞组和溶剂对照组（P〈0.01）。治疗后4,7,14d骨髓间充质干细胞组脑组织中肝细胞生长因子的表达水平显著高于大脑中动脉栓塞组和溶剂对照组（P〈0.01）。提示骨髓间充质干细胞移植可促进大鼠缺血区微血管生成,改善缺血区血运,从而改善脑缺血大鼠的神经功能。

Effects of bone marrow mesenchymal stem cells transplantation on angiogenesis and hepatocyte growth factor expression in the rat brain after focal cerebral ischemia

BACKGROUND： Bone marrow mesenchymal stem cells （BMSCs） transplantation can improve neurological function in cerebral ischemic rats. However, few studies have reported this mechanism. OBJECTIVE： To probe into the mechanisms of BMSCs transplantation on the recovery of neurological functions in the rat brain after focal cerebral ischemia. METHODS： Male adult Sprague-Dawley rats were randomly assigned into 4 groups： sham-operated group, middle cerebral artery occlusion （MCAO） group, vehicle group and MCAO＋BMSCs group. Permanent focal cerebral ischemia models were established using the modified Longa＇s method. BMSCs were injected into the lateral ventricle of MCAO＋BMSCs group one day after right MCAO, and the same dose of PBS was given to the vehicle group. RESULTS AND CONCLUSION： A lot of microvessels proliferated in the injured cortex, reaching peak at 2 weeks. The microvessel density in the injured cortex of rats treated with BMSCs was higher than that of the MCAO group and the vehicle group （P 0.01）. The expression of hepatocyte growth factor （HGF） in brain tissues in the MCAO＋BMSCs group was higher than that in the MCAO group and the vehicle group at 4, 7, 14 days after treatment （P 0.01）. These data suggest that BMSCs transplantation can improve neurological function in cerebral ischemic rats through promoting revascularization in the injured region.