Establishment of a monoclonal antibody directed to the minor novel gangliosides in bovine brain and cultured neural cell lines |
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| Authors: | H Yamamoto S Tsuji Y Nagai |
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| Affiliation: | Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan. |
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| Abstract: | We established a MAb N-25 reacted with a minor unknown antigen (AgX) in a commercially available GQ1b sample. It also recognized minor antigens in bovine brain (X-1, 2, 3, 4 and 5) and cultured neural cell lines (X-1). AgX is identical to X-5. X-5 is sialidase sensitive and has the common structure as X-1, which is resorcinol positive. These results suggested that novel gangliosides exist in bovine brain and neural cell lines. |
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