首页 | 本学科首页   官方微博 | 高级检索  
     


Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains
Authors:Rossi S L  Nasar F  Cardosa J  Mayer S V  Tesh R B  Hanley K A  Weaver S C  Vasilakis Nikos
Affiliation:
  • a Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0610, USA
  • b Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX 77555-0610, USA
  • c Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX 77555-0610, USA
  • d Institute of Health & Community Medicine, Universiti Malaysia Sarawak, Kota Samarahan, Sarawak, Malaysia
  • e Department of Biology, New Mexico State University, Las Cruces, NM 88005, USA
  • Abstract:
    Four serotypes of dengue virus (DENV 1-4) currently circulate between humans and domestic/peridomestic Aedes mosquitoes, resulting in 100 million infections per year. All four serotypes emerged, independently, from sylvatic progenitors transmitted among non-human primates by arboreal Aedes mosquitoes. This study investigated the genetic and phenotypic changes associated with emergence of human DENV-4 from its sylvatic ancestors. Analysis of complete genomes of 3 sylvatic and 4 human strains revealed high conservation of both the 5′- and 3′-untranslated regions but considerable divergence within the open reading frame. Additionally, the two ecotypes did not differ significantly in replication dynamics in cultured human liver (Huh-7), monkey kidney (Vero) or mosquito (C6/36) cells, although significant inter-strain variation within ecotypes was detected. These findings are in partial agreement with previous studies of DENV-2, where human strains produced a larger number of progeny than sylvatic strains in human liver cells but not in monkey or mosquito cells.
    Keywords:Dengue virus (DENV)   Sylvatic DENV   Human DENV   Phylogenetic and phenotypic analyses
    本文献已被 ScienceDirect PubMed 等数据库收录!
    设为首页 | 免责声明 | 关于勤云 | 加入收藏

    Copyright©北京勤云科技发展有限公司  京ICP备09084417号