从缺血的病理生化改变探讨神经元损伤的机理

目的：探讨缺血性神经元损伤的机理。方法：用体培养海马神经元建立缺血模型，通过检测神经元致死率以及观察由缺血引起的细胞内生化代谢改变所致的神经元功能障碍，探讨缺血性神经元损伤的机理，结果：发现缺血组、缺葡萄糖组元致死率最高，且通过检测细胞膜表面磷脂酰丝氨酸的变化证实此二组神经元凋亡率也最高。缺血可引起元细胞骨架结构改变，使神经元功能遭到破坏，最终导致神经元不可逆损伤。结论：缺血的是生化改变通过不同的

Study on mechanisms of hippocampal neuronal damage induced by ischemia in vitro

objective: The present study aims to prove the mechanisms of ischemic neuronal damage byresearch the pathobiochemical changes during ischemia. Methods :The present study made the ischemic model in thecultural hippocampal neurons. We measured the death rate of neurons and apoptotic neurons by means of Annexin- V and propidium iodine (PI)immunofluorescent double-staining method. Cytoskeleton morphorlogy was observedby neurofilament(NF200)immunofluorescent staining. Results: Our study indicated that ischemic-like treatmentled to most severe neuronal death and apoptosis in cultural neurons. It was followed by a group of absence of glucose. The mild apoptotic neuronal death was presented in glutamate, hypoxia and anoxia groups. The cytoskeleton morphology was obviously changed from normal tl disorder amd breakage. The meurofilament clustered in disorder and formed clusters in induced neurons. Conclusion:The ischemia results in neuronal damage by means of variors mechanisms.[