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| 慢性丙型肝炎患者外周血单个核细胞中负链HCV RNA的检测及意义 | |
| 引用本文： | 白雯,聂青和,胡大荣,陈国致,李梦东. 慢性丙型肝炎患者外周血单个核细胞中负链HCV RNA的检测及意义[J]. 实用肝脏病杂志, 1998, 0(2) |
| 作者姓名： | 白雯 聂青和 胡大荣 陈国致 李梦东 |
| 作者单位： | 西安市 解放军323医院检验科 710054，第三军医大学西南医院全军传染病中心，第三军医大学西南医院全军传染病中心，第三军医大学西南医院全军传染病中心，第三军医大学西南医院全军传染病中心 |
| 摘 要： | 19例临床诊断慢性丙型肝炎患者,检测其外周血单个核细胞中丙型肝炎病毒(HCV)RNA的存在及复制状态。所选检测对象血清标本抗-HCV及HCV RNA均为阳性。采用逆转录-套式PCR法检测其外周血单个核细胞中HCV正、负链RNA,结果19例患者外周血单个核细胞中14例HCV正链RNA阳性;6例HCV负链RNA阳性。实验结果证实部分慢性丙型肝炎患者外周血单个核细胞中存在HCV复制,表明肝细胞并非为HCV感染与复制的唯一场所。HCV感染慢性化与其外周血单个核细胞中存在HCV复制可能有一定关系。 |
| 关 键 词： | 丙型肝炎病毒 外周血单个核细胞 核糖核酸 逆转录-多聚酶链反应 |
DETECTION AND IMPLICATION OF THE MINUS STRAND HCV RNA IN PBMC OF PATIENTS WITH CHRONIC HEPATITIS C |
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| Bai Wen,Nie Qinghe,Hu Darong,et al.. DETECTION AND IMPLICATION OF THE MINUS STRAND HCV RNA IN PBMC OF PATIENTS WITH CHRONIC HEPATITIS C[J]. Journal of Clinical Hepatology, 1998, 0(2) | |
| Authors: | Bai Wen Nie Qinghe Hu Darong et al. |
| Affiliation: | Bai Wen,Nie Qinghe,Hu Darong,et al.323 rd Hospital of PLA,Xi'an,710054 |
| Abstract: | Peripheral blood mononuclear cells (PBMC)from 19 patients with chronic hepatits C were collected and detected for the presence of replicative forms of hepatitis C virus (HCV).HCV RNA in PBMC was extracted and tested for both plus and minus strand by RT-PCR.All of these serum were anti-HCV and HCV RNA positive.The results showed that the plus strand HCV RNA was found in PBMC of 14 patients and the minus strand of HCV RNA in PBMC of 6 patients.These results suggest that HCV does replicate in the PBMC in part of patients with chronic hepatitis C. |
| Keywords: | Hepatitis C Virus Peripheral Blood Mononuclear Cells RNA. |
| 本文献已被 CNKI 等数据库收录！ | |