早期复合营养减轻湿热创伤大鼠应激损伤的研究

目的 探讨早期肠内复合营养对高温高湿刨伤复合应激条件下大鼠肛温、NO、SOD、MDA的影响,为研究提高应对应激反应的能力提供实验依据.方法 将56只湿热创伤大鼠模型随机分为对照组、营养组各28只,每组各分为热应激1,2 h、热应激后4,10 h 4个时相点,每个时相点7只大鼠.对照组予去离子水灌胃1周,99℃沸水背部浅Ⅱ度烫伤,烫伤面积约为体表面积的10%;营养组动物给予复合营养素灌胃1周后施以对照组同样的创伤处理,2组动物均置于干球温度(37.0+0.5)℃、相对湿度(65±5)%的湿热环境中.比较4个时相点2组大鼠的肛温、NO、SOD及MDA情况..结果 对照组和营养组4个时相点间肛温、NO、SOD含量差异明显.结论 早期肠内给予复合营养素可以减轻直肠温度的升高程度以及急性应激反应中NO、SOD、MDA浓度和对机体的影响水平,从而降低机体的代谢反应.

The research of compound nutrition to reduce stress damage in moist heat and wound rats

Objective To probe into the changes of rats' plasma SOD,MDA,NO and rectal temperature at moist heat and wound stress in order to build a basis for research of enhancing resistance to stress resporise in human. Methods 56 moist beat and wound rats were randomly divided into the control group and the nutrition group with 28 rats in each group.then each group was subsequently divided into 4 period oftime.All the backs of rats were scalded in superficial degree Ⅱ by 99℃water and exposed to the same conditions:temperature at(37.O±0.5)℃and relative humidity at(65±5)%.The control group' was imbued with double-distilled water for one week,while in the nutrition group,compound nutrient was imbued for one week.SOD,MDA,NO and rectal temperature at 4 time points were compared between the two groups.Results The differences of rectal temperature,NO and SOD changes between the control group and the nutrition group were remarkably significant.Conclusions Early nutrition is effective in reducing stress,decreasing organ injury caused by NO,SOD and MDA level and alleviated the damage of body.