Wnt3a基因沉默对内皮祖细胞增殖的影响

目的 探讨wnt3a基因沉默对内皮祖细胞( EPCs)增殖的影响. 方法 体外分离、培养及鉴定EPCs. 将沉默型pEGFP-shRNA-wnt3a重组质粒转染EPCs; Western blot法分析wnt3a 在 EPCs 中的表达变化;MTT 分析 wnt3a 沉默对EPCs增殖的影响. 结果 鉴定培养的细胞为EPCs;Western blot法结果提示pEGFP-shRNA-wnt3a转染EPCs后明显抑制了 wnt3a 蛋白的表达. MTT 提示沉默型 pEGFP-shRNA-wnt3a转染组吸光度值明显低于空白对照组. 结论 成功分离、培养出EPCs,wnt3a有效沉默明显抑制EPCs的增殖,为进一步的实验提供了基础.

Effects of wnt3a silencing on the proliferation of endothelial progenitor cells

Objective To observe the effects of wnt3 a silencing on the proliferation of endothelial progenitor cells ( EPCs) . Methods We used the mature methods of isolation, culture and identification on cells to get EPCs in vitro. The endothelial progenitor cells were transfected with the the silent type pEGFP-shRNA-wnt3a recombinant plasmid. The expression changes of wnt3a were detected by Western blot. MTT assay was used to assess the effects of wnt3a silencing on the proliferation of EPCs. Results The cultured cells were confirmed to be EPCs. The re-sults of Western blot revealed that wnt3 a expression was obviously inhibited in transfected EPCs with pEGFP-shR-NA-wnt3a. MTT assay showed that the proliferation of EPCs was significantly inhibited by effective silencing of wnt3a as compared with the control group. Conclusion We have successfully isolated and cultured EPCs in vitro. Wnt3a effectively silence inhibits the proliferation of EPCs. And this study provides the basis for further experi-ments.