颅脑创伤后大鼠脑组织中Apelin表达变化的研究

目的研究颅脑创伤后大鼠脑组织中Apelin表达的变化规律。方法 96只雄性SD大鼠随机分为假手术组(A组)、轻型颅脑创伤组(B组)和重型颅脑创伤组(C组),每组又分6小时、24小时、72小时、168小时4亚组,RT-PCR法观测Apelin的基因表达,免疫组化法观测Apelin的表达。结果 B组和C组的Apelin表达高于A组,并在24小时达到高峰,而且在C组的升高程度大于B组。结论颅脑损伤后脑组织中Apelin的表达增加,并且与损伤严重程度相关。

Changes of Apelin in the rat after traumatic brain injury

Objective To study the changes of Apelin in the rat after traumatic brain injury. Methods 96 male SD rats were randomly divided into 3 groups, namely the sham operation group（group A）, the mild traumatic brain injury group（group B） and the severe traumatic brain injury group（group C）. Each group was further divided into four sub-groups according to the time interval between injuries and scarify of the rats. The ex-pression of Apelin in the brain tissue was detected by immunohistochemical technique and RT-PCR. Results The expression of Apelin in the group B and C were both significantly higher than that in the group A（P〈0.05）, It reached a peak 24 h after injury. Furthermore it had higher level in group C than that in group B（P〈0.05）. Conclusion The results suggested that Apelin was up-regulated obviously in the brain after traumatic brain injury, and it was assosiated with injury severity.