酒精性脂肪肝大鼠肠源性内毒素血症模型的建立

目的：建立酒精性脂肪肝大鼠肠源性内毒素血症模型。方法采用梯度酒精灌胃法早晚两次灌胃，并以10%酒精为饮料，建立大鼠酒精性脂肪肝模型。分别于3w和6w检测肝脂肪变、肝内炎症、肝功能和血清内毒素水平。结果模型组自6w出现显著的肝脂肪变，肝指数（3.6±0.2）、肝脂肪变积分（3.0±0.9）和炎症积分（1.0±0.6）均显著高于同期对照组水平分别为（3.1±0.1）、（0.0±0.0）和（0.0±0.0），P＜0.05]；在6周时模型动物血浆内毒素、血清D-乳酸、二胺氧化酶和AST分别为（1435.6±52.9）pg/ml、（20.7±5.4）mmol/L、（25.5±2.0）U/L和（124.5±13.2） U/L，较正常组均明显升高分别为（89.9±10.5）pg/ml、（5.0±1.1）mmol/L、（7.4±1.7）U/L和（40.4±15.2）U/L，P＜0.05]。结论用该方法连续6w成功建立单纯性酒精性脂肪肝肠源性内毒素血症模型。

Establishment of experimental model of alcoholic fatty liver accompanied with intestinal endotoxemia in rats

Objective To establish an experimental model of alcoholic fatty liver accompanied with intesti-nal endotoxemia in rats. Methods A rat model of alcoholic fatty liver was established by gradient ethanol gavage method;The liver steatosis,intrahepatic inflammation,liver function tests and serum lipopolysaccharide were deter-mined at 3 and 6 weeks of experiment. Result Significant liver steatosis was observed after 6 weeks of ethanol administration in the model rats;The liver index,liver steatosis scores,inflammation scores in model rats were（3.6± 0.2）,（3.0±0.9）and （1.0±0.6）,respectively, significantly higher than those in normal controls （3.1±0.1）,（0.0±0.0） and （0.0 ±0.0）,respectively,P＆lt;0.05）];Similarly,serum lipopolysaccharide,D-lactate,diamine oxidase and aspertate aminotransferase levels in model rats at 6 months were （1435.6 ±52.9） pg/ml,（20.7 ±5.4） mmol/L,（25.5 ±2.0）U/L and （124.5±13.2） U/L,which were also significantly higher than those in normal controls（89.9±10.5） pg/ml,（5.0± 1.1）mmol/L,（7.4±1.7） U/L and（40.4±15.2） U/L,respectively,P＆lt;0.05]. Conclusion A rat model of alcoholic fatty liver accompanied with intestinal endotoxemia can be successfully establish by using gradient ethanol gavage for 6 weeks.