精液的两种不同处理方式对体外受精-胚胎移植的影响

目的：探讨精液的两种不同处理方式对体外受精-胚胎移植的影响。方法：对73例继发不育的IVF—ET患者的结局进行回顾性分析。根据进入周期精液处理方式的不同将患者分为2组：梯度离心法处理组（A组）42例；梯度离心上游法联合应用组（B组）31例。结果：两组的促性腺激素用量、促排天数、获卵数、受精率、卵裂率、优质胚胎率间差异无统计学意义（P〉0．05），胚胎种植率和临床妊娠率A组低于B组，A组分别为18．2％（16／88），26．2％（11／42）；B组2．3（20／62），51．6％（16／31），两组的种植率和临床妊娠率相比较有统计学意义（P〈0．05）。结论：梯度离心法和上游法联合应用处理精液比单纯应用梯度离心法处理精液有助于提高IVF—ET患者的种植率和临床妊娠率，可改善妊娠结局。

Effects of the semen separate methods on the clinical outcome of in vitro fertilization and embryo transfer.

Objective：To investigate the relationship of the semen separate methods and the clinical outcome oi in vitro fertilization and embryo transfer（IVF-ET）. Methods：Seventy three cycles of IVF-ET with second infertility were analyzed retrospectively. These cycles were divided into two groups on the basis of semen separate methods：group A （Isolate）42 cycles, group B（Isolate and up swim）31 cycles. Results： There was no significant difference in the the dosage of gonadotrophins（Gn）, the days of Gn, the number of oocytes retrieved, the fertilization rate, the cleavage rate and high-quality embryo rate（P〈0. 05）, the embryo implantation rate and pregnancy rate of group A was lower than group B（P〈0.05）, group A 18. 2% （16/88）, 26. 2 % （ 11/42 ）, group B 2.3 （ 20/62）, 51.6 % （ 16/31 ）. Conclusions： The clinical outcome of in vitro fertilization and embryo transfer with semen separate methods of Isolate and up swim is better than separate methods of Isolate.