重组人碱性成纤维细胞生长因子对大鼠动脉内皮损伤后再狭窄的影响

目的：探讨重组人碱性成纤维细胞生长因子（rh-bFGF)对经皮冠状动脉腔内成形术后再狭窄的预防效果。方法：用2F球囊导管造成大鼠左颈总动脉内皮损伤。治疗组每天im rh-bFGF 10kU/kg.分别于术后7天和14天，每组各处死大鼠10只，取左颈总动脉进行[^3H]胸腺嘧啶掺入测定和病理形态学检查。结果：在第7天和第14天时，与模型对照组相比，rh-bFGF治疗组颈总动脉平均新生内膜厚度明显变薄；平均中膜面积缩小；平滑肌细胞和弹力板层数减少；胶原含量及[^3H]胸腺嘧啶掺入量也比模型对照组明显降低。结论：适当应用rh-bFGF有抑制气囊损伤后动脉新生内膜增厚，降低再狭窄发生的作用。

Effects of recombinant human basic fibroblast growth factor on restenosis after arterial endothelial injury in rats

AIM: To study the preventive effect of recombinant human basic fibroblast growth factor (rh-bFGF) on restenosis after percutaneous transluminal coronary angioplasty (PTCA). METHODS: Sixty male Wistar rats were randomly divided into sham operation group, intima injured group, and intima injured plus rh-bFGF treated group. Rat carotid arteries were injured using a balloon catheter except sham operation group. The rats of rh-bFGF treated group were injected im rh-bFGF 10 kU . kg-1 . d-1 after intima was injured. Ten rats in each group were killed on d 7 and d 14 after injury, respectively. [3H]Thymidine incorporation assay and pathological examination were carried out to each vessel. RESULTS: (1) Seven days after injury, the average intimal thickness in sham operation group, intima injured group, and rh-bFGF treated group was (7 +/- 1), (32 +/- 11), and (17 +/- 3) micron; average intimal area was (384 +/- 145), (1530 +/- 817), and (586 +/- 185) micron2; the numbers of smooth muscle cells in neointima per transect were 0 +/- 0, 146 +/- 18, and 105 +/- 26; the ratio of the collagen area to intimal area plus medial area were 0.29 +/- 0.09, 0.7 +/- 0.3, and 0.30 +/- 0.14; [3H]thymidine incorporation were (17 +/- 6), (62 +/- 23), (20 +/- 8) kBq/g tissue, respectively. (2) Fourteen days after injury, the average intimal thickness of sham operation group, intima injured group, and rh-bFGF treated group was (8 +/- 1), (41 +/- 9), and (20 +/- 3) micron; average intimal area was (391 +/- 134), (1761 +/- 337), and (731 +/- 124) micron2; the numbers of smooth muscle cells in neointima per transect were 0 +/- 0, 145 +/- 9, and 102 +/- 6; the ratio of collagen area to intimal area plus medial area were 0.28 +/- 0.14, 0.59 +/- 0.21, and 0.38 +/- 0.03; [3H]thymidine incorporation was (15 +/- 4), (57 +/- 11), and (22 +/- 6) kBq/g tissue, respectively. CONCLUSION:Large dosage of rh-bFGF inhibits neointimal hyperplasia and reduces restenosis after balloon injury.