应用PCR技术检测慢性乙肝病毒性肝病血清及外周血单个核细胞中HBV－DNA

本文应用多聚酶连反应（ＰＣＲ）技术检测了１０４例慢性乙肝病毒性肝病患者血清及外周血单个核细胞（ＰＢＭＣ）中ＨＢＶ－ＰＮＡ，并与放免法进行了比较。结果显示，ＰＣＲ检测结果更能客观地反应ＨＢＶ在体内存在的状态，在外周血单个核细胞中有ＨＢＶ感染；１０４例慢性肝病患者外周血单个核细胞中ＨＢＶ－ＤＮＡ的检出率为２８．８％，而血清中ＨＢＶ－ＤＮＡ阴性的２０例患者有７例ＰＢＭＣ中检测出ＨＢＶ－ＤＮＡ，占３５％。因此，在检测血清ＨＢＶ标志物及ＨＢＶ－ＤＮＡ的同时检测ＰＢＭＣ中的ＨＢＶ－ＤＮＡ可进一步防止ＨＢＶ传播与输血后肝炎的发生。

Detection of HBV-DNA in serum and PBMC of the patients with chronic HBV hepatic disease by PCR

HBV-DNA in the serum and peripheral blood mononucleated cells(PBMC)of the104 patients with chronic HBV hepatic disease was detected by PCR and compared with results by ra- dio-immunoassay. The results showed that the method by PCR could even more objectively reflectHBV condition kept in body and there was infection in PBMC. Positive rate of HBV-DNA in PBMCof l04 patients with chronic HBV hepatic disease detected by PCR was 28.8%. In 20 HBV-DNAnegative patients there were 7 patients whose HBV-DNA in PBMC (35%)was positive. Therefore,detecthg HBV-DNA in PBMC and determing HBV marker and HBV-DNA in serum could si-multaneously check the spread of HBV and prevent hepatitis after transfusion.