姜黄素预处理对体外循环大鼠心肌缺血再灌注损伤的保护与抗氧化作用

目的:探讨姜黄素预处理对体外循环期间大鼠心肌缺血再灌注引起的氧化应激损伤的保护作用.方法:将90只SD大鼠随机分为6组,每组15只,分别为假手术组,缺血再灌注损伤(MIR)组,溶剂组,姜黄素低、中、高(10,20,40 mg·kg-1)剂量组.建立心肌缺血再灌注模型.用不同剂量姜黄素在人为制造大鼠心肌缺血模型前30 min分别做预处理.再灌注结束后采血,测定丙二醛(MDA)、超氧化物歧化酶(SOD)、天门冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、乳酸脱氢酶同工酶( LDH1),并测量心肌梗死面积.结果:姜黄素预处理能明显降低血浆中AST,LDH,LDH1,MDA的含量,MIR组血浆中各种心肌酶均明显高于假手术组,差异有统计学意义(P＜0.05),MIR组与溶剂组比较差异无统计学意义,姜黄素组血浆各心肌酶均明显低于MIR组,差异有统计学意义(P＜0.05),提高SOD活性,减少心肌梗死面积.结论:姜黄素对体外循环期大鼠心肌缺血再灌注引起的氧化应激损伤有保护作用.

Protective Effects of Curcumin on Myocardial Ischemia Reperfusion Injury in Rats in Cardz Pulmonary Bypass

Objective: Toinvestigate the protective effect of curcumin on oxidative stress injury induced by myocardial ischemia reperfusion(MIR) in rats in cardz pumonary by pass(CPB). Method: Sixty healthy SD rats were randomized in to six groups:shamgroup,MIR group,PEG group,MIR+high dose curcumin treatment group,MIR+ middle dose curcum in treatmen tgroup,MIR+low dose curcum in treatment group.My ocardial IR was carried out by ligation of left anterior descending coronary artery for 30 min followed by reperfusion for 60 min.Dif-ferent dosages of curcumin were administered 30 min before the ligation.Blood was collected at the end of reperfusion for detecting changes of aspartate aminotransferase(AST),lactate dehydrogenase(LDH),lactate dehydrogenase1(LDH1),superoxide dismutase(SOD),and malondialdehyde(MDA).The myocardial infarct size was also estimated. Result: Curcumin pretreating dose-depend-ently decreased the contents of AST,LDH,LDH1 and MDA,and the myocardial infarct size,and increased the activity of SOD with dose-dependent manner. Conclusion: Curcum in exerts protective effects on myocardial is chemia reperfusion injury.