骨碎补与夜交藤组合物对α-糖苷酶活性的影响

目的:研究骨碎补与夜交藤组合物对α-糖苷酶活性的影响.方法:设酶、骨碎补与夜交藤组合物的提取物、拜唐苹阳性药和空白对照,以及中药组合物的提取物空白对照.把阳性药和以上中药组合物的提取物,加入到α-淀粉酶和α-麦芽糖酶不同微量反应体系中,分别用Bernfeld法、葡萄糖氧化酶(GOD)法测其酶活性.结果:骨碎补与夜交藤组合物的水提物和浸膏对α-糖苷酶中的α-麦芽糖酶活性均有明显的抑制作用,与拜唐苹的作用一致,其抑制存在量效关系,但呈可逆性、混合性抑制.结论:骨碎补与夜交藤组合物的水提物、浸膏是对α-糖苷酶起抑制作用的主要部位.

Effect of the Combination of Drynaria fortunei and Ploygonum multiflorum on Alpha-glycosidase Activity

Objective:To investigate effects of the combination of Drynaria fortunei and Ploygonum multiflorum on alpha-glycosidase activity.Method: Following groups including enzyme,the combination of extracts of D.fortunei and P.multiflorum,acarbose and the positive drug,blank control and controls of the herbal extracts were established.The activity of different micro reaction systems like α-amylase or α-maltase,which were added to acarbose and the above correspoding groups,was determined by Bernfeld,and GOD.Result: The aqueous extract,sodden ointment of the combination of D.fortunei and P.multiflorum showed significant inhibition on α-maltase’s activity with dose-effect relationship,consistent with the role of acarbose,but the inhibition was reversible and mixed.Conclusion: The aqueous extract and sodden ointment of D.fortunei and P.multiflorum combination have inhibition on α-glycosidase.