大鼠自身免疫性葡萄膜视网膜炎辅助T细胞的类细胞因子水平表达

目的观察实验性自身免疫性葡萄膜视网膜炎（experimental autoimmune uveoretinitis，EAU）大鼠血清以及脾细胞培养上清液中辅助T细胞1／辅助T细胞2（helper T cell 1／helper T cell2，Th1／Th2）类细胞因子水平。方法用Fmoc法合成光感受器间维生素A类结合蛋白R16多肽片段，联合免疫佐剂诱导EAU动物模型。在EAU高峰期取大鼠血清，分离脾细胞，培养后取上清液，应用酶联免疫吸附法检测血清以及脾细胞培养上清液中Th1类细胞因子（interferon-γ，IFN-γ、interleukin-2，IL-2）和Th2类细胞因子（IL-4、IL-10）水平。结果EAU组大鼠血清中IFN-1和IL-2的浓度分别为（33．8±5．2）μg／L，（52．5±7．9）μg/L，显著高于空白对照组[（6．2±1．4）μg/L，（3．7±0．8）μg/L和弗氏完全佐剂对照组[（complete Freund＇s adjuvant，CFA）；（9．2±1．9）μg/L，（5．1±1．1）μg/L]（P〈0．05）；而IL-4、IL-10的浓度与空白对照组和CFA组相比差异无统计学意义。EAU组大鼠脾细胞培养上清液中IFN-γ、IL-2的浓度分别为（1105．3±197．5）μg/L，（45．0±16．2）μg/L，显著高于空白对照组（5．24±1．7）μg/L，（4．1±1．3）μg/L和CFA组（25．14±5．9）μg/L，（5．1±1．9）μg/L（P〈0．01），IL4、IL-10的浓度与空白对照组和CFA组相比差异无统计学意义；CFA组大鼠脾细胞培养上清液中IFN-1的浓度明显高于空白对照组（P〈0.05），IL-2、IL-4、IL-10的浓度与空白对照组相比差异无统计学意义。结论在EAU高峰期，Th1类细胞因子水平显著升高，提示EAU是Th1细胞诱导的疾病（中国眼耳鼻喉科杂志，2008，8：280-282）

Helper T cell cytokines expression in autoimmune uveoretinitis in rats

Objective To investigate the expression of helper T cell 1/helper T cell 2（Th1/Th2） cytokines in serum and culture supernatants of splenic cells in rats with experimental autoimmune uveoretinitis （EAU）. Methods Fmoc procedure was used to synthesize the interphotoreceptor retinoid-binding protein （IRBP） R16 peptide in order to establish EAU animal model combined with immune adjuvant. The expression of interferon-γ（ IFN-γ）, interleukin-2 （IL-2）, IL-4 and IL-10 in serum, culture supernatant of splenic cells was determined by enzyme-linked immunosorbent assay kits. Results The serum levels of IFN-γ and IL-2 in EAU rats were （33.8 ± 5.2） μg/L and （52.5 ± 7.9 ） μg/L respectively, which were significantly higher than those in the control group [ （6.2 ± 1.4 ） μg/L, （3.7 ±0.8） μg/L] and complete Freund＇s adjuvant（CFA） group [ （9.2 ± 1.9 ） μg/L, （5.1 ± 1.1 ） μg/L] （P 〈0.05）. The serum levels of IL-4 and IL-10 in EAU rats showed no significant differences compared with the control or CFA group. The levels of IFN-3, and IL-2 in the culture supernatant of splenic cells in EAU rats were （ 1 105.3 ± 197.5 ） μg/L and （45.0 ± 16.2） μg/L, which were significantly different from those in the control group[ （5.2 ± 1.7） μg/L, （4.1 ± 1.3 ）μg/L] ） and CFA group [ （25.1 ±5.9 ） μg/L, （ 5.1 ± 1.9） μg/L] ] （P 〈0.01 ）. The levels of IL-4 and IL-10 were not significandy different from those in the control and CFA group. IFN-γlevel in the culture supernatant of splenic cells in CFA rats was significantly higher than those in the control group （P 〈 0.05 ） while the levels of IL-2, IL-4, IL-10 were not significantly different between the two groups. Conclusions An increased production of Th1 cytokine, IFN-γ and IL-2 was observed in the EAU rats, suggesting that EAU may be mainly induced by Th1 cells. （ Chin J Ophthalmol and Otorhinolaryngo1,2008,8：280-282）