免疫性血小板减少性紫癜患者CD4~＋CD25~（high）CD127~（dim）调节性T细胞检测及其意义

目的探讨调节性T细胞（Treg）在ITP患者发病中的作用机制及其与PAIgG之间的相关性。方法采用流式细胞术（FCM）分别检测ITP患者和健康体检者外周血CD 4＋CD 25highCD 127dimTreg细胞占CD 4＋T细胞比例及PAIgG的表达水平。结果 ITP组CD 4＋CD 25highCD 127dimTreg细胞比例较正常对照组明显下降（P〈0.05）;ITP组PAIgG表达水平明显高于正常对照组（P〈0.05）;Treg细胞与PAIgG之间呈负相关（r=-0.57,P〈0.05）。结论 ITP患者CD 4＋CD 25highCD 127dimTreg细胞水平显著下降,免疫抑制作用减弱,引起自身反应性T、B细胞活化增殖,T细胞亚群改变,PAIgG产生增加,并最终导致ITP发病。

Detection and Significance of CD 4+CD25highCD127dim Regulatory T Cells in Patients with Immune Thrombocytopenic Pupura

Objective To evaluate the role of CD 4+CD 25highCD 127dim regulatory T cells(Treg) in ITP and the association with PAIgG.Method With FCM,we examed the pecentage of CD 4+CD 25highCD 127dim regulatory T cells in CD 4+T cells and the level of PAIgG in peripheral blood.Results The pecentage of Treg in peripheral blood of patients with ITP was significantly lower than that in healthy control group(P<0.05);The level of PAIgG in patients group was higher than that in healthy control group(P<0.05);The pecentage of Treg cells was negatively correlated with the level of PAIg(r=-0.57,P<0.05).Conclusion In patients with ITP.The pecentage of Treg reduced,effection of immunosuppression abated,autoreactive T,B cells activated and proliferated,T cell subsets changed,the production PAIgG increased,eventually led to the onset of ITP.