片仔癀对人结肠癌SW480细胞株的抑制作用研究

目的探究片仔癀对人结肠癌SW480细胞株的抑制效果。 方法通过对人结肠癌SW480细胞株常规体外培养，随机设定空白组、5-氟尿嘧啶（5-FU）组和不同浓度片仔癀组，分别采用对应药物干预24 h、48 h、72 h，并通过细胞增殖活力检测法（MTT法）和荧光显微镜观察其抑制效果。采用人结肠癌SW480细胞株建立结肠癌小鼠模型，把造模成功的150只小鼠随机分为模型组，5-FU组和片仔癀低、中、高剂量组，每组30只；分别以不同剂量药物外敷3 d，通过抑瘤率和原位凋亡检测（TUNEL法）解释抑制效果。 结果1 mg/mL、2 mg/mL、3 mg/mL浓度片仔癀对人结肠癌SW480细胞体外抑制率最高，为70.05%、71.39%、70.12%，与5-FU组比较差异具有统计学意义（χ²=4.49，4.97，4.52；均P<0.05）。4 mg/mL片仔癀抑制率为67.39%，与5-FU组比较差异无统计学意义（χ²=3.57，P>0.05）；荧光显微镜显示5-FU组与片仔癀各组人结肠癌SW480细胞株数量均显示不同程度的减少、体积变小、折光性差、细胞悬浮及脱落死亡。片仔癀高、中、低剂量组对人结肠癌SW480细胞的体内抑瘤率分别为51%、39%、23%，其中高、中剂量组与5-FU组比较差异无统计学意义（χ²=0.05，0.49；均P>0.05），低剂量组与之比较差异有统计学意义（χ²=4.09，P<0.05）；荧光显微镜显示片仔癀各剂量组和5-FU组对人结肠癌SW480细胞均有不同程度的凋亡反应。 结论片仔癀外用对人结肠癌SW480细胞生长有明显的抑制作用，高、中剂量体内抑制作用与5-Fu相近，优于低剂量，临床可考虑采用高剂量可能取得更佳效果，体外抑制作用上显示则优于5-Fu，但剂量差异影响不大。

Study of inhibitory effect of Pien Tze Huang on human colon cancer SW480 cell line

ObjectiveTo explore the Pien Tze Huang(PTH) inhibitive effect of human colon cancer SW480 cell line. MethodsThroug the human colon cancer SW480 cell line in vitro cultivation, we randomly set five group, which was blank group, 5-FU group and others in the differernt doses of PTH, respectively adopt the corresponding drug intervention for 24 h, 48 h, 72 h, then use the cell proliferation assay (MTT method) and fluorescence microscope to observe the inhibition effect. The animal model was established by using SW480 human colon cancer cells, and we randomly divided 150 mice into model group, 5-FU group and PTH low, medium and high dose group, each group of thirty. To explain the inhibitory effect by anti-tumor rate and in situ apoptosis (TUNEL method) .We use three days of external drug intervention. ResultsThe inhibitory rates of 1 mg/mL, 2 mg/mL and 3 mg/mL PTH on human colon cancer SW480 cells in vitro were 70.05%, 71.39% and 70.12%, which were significantly different from those in 5-FU group (χ²=4.49, 4.97, 4.52; all P<0.05). The inhibition rate of 4 mg/mL tablets was 67.39%. There was no significant difference between 5-FU group and 5-FU group (χ²=3.57, P>0.05). Fluorescence microscopy showed that the number of SW480 cell lines in 5-FU group and 5-FU group decreased in varying degrees, with smaller volume, poor refractive index, cell suspension and exfoliation death. The inhibition rates of high, medium and low dose groups of PTH on SW480 cells were 51%, 39% and 23% respectively. There was no significant difference between high and medium dose groups and 5-FU group (χ²=0.05, 0.49; all P>0.05), but there was significant difference between low dose group and low dose group (χ²=4.09, P<0.05). Each dose group and group 5-FU had different degrees of apoptosis on human colon cancer SW480 cells. ConclusionPTH have significant inhibitive effect on human colon cancer SW480 cell in vivo, and the inhibitory effect of high and middle doses is similar to that of 5-FU, which is better than low dose. Therefore, the effect of high dose could be considered better, and the inhibitory effect in vitro is better than that of 5-FU, but the effect of dose difference is not significant.