反向点杂交法测定浙江省4620例男性HPV感染者的流行病学特征

目的调查浙江省男性人乳头瘤病毒(human papilloma virus，HPV)感染的亚型分布及流行病学特点，为男性HPV感染早期筛查、预防、诊断提供依据。 方法回顾性汇总分析2014年1月至2016年12月期间，浙江省17~74岁的4620名男性尖锐湿疣皮损拭子或组织标本；4620例样本中，其中120份因细胞量不足判定采样失败，剩余4500例样本按不同年龄分为6组(17~20岁组268名、21~30岁组1958名、31~40岁组1156名、41~50岁组667名、51~60岁组355名、>60岁组96名)。用聚合酶链反应(polymerase chain reaction, PCR)和反向点杂交法测定23种HPV基因型，并分析其HPV阳性率分布、HPV阳性率差异和高低危型HPV感染特征。 结果4500例样本中有2799例HPV阳性，总阳性率为62.2%，其中：以6型和11型阳性率最高，分别为15.58%(436/2799例)和10.00%(280/2799例)。高危型阳性率最高的为52型，占4.72% (132/2799例)。在2799例HPV DNA阳性者中，有1266例为单一感染；混合感染1533例，占44.26%，单纯低危型最高为三重感染，单纯高危型最高为四重感染，而高低危混合感染达七重，其中七重感染占2.25%(63/2799例)。高低危混合感染数高于单纯高危型和单纯低危型，两者比较有统计学差异(χ²分别为28.1、82.8，P均<0.05)。HPV阳性结果以21~40岁青壮年为主，占总人数的69.2% (3114/4500)。而HPV阳性率以17~20岁组(75.0%)和51~60岁组(79.7%)最高，与其他年龄段21~30岁(60.7%)、31~40岁(59.1%)、41~50岁(59.6%)和>60岁(46.4%)组]相比χ²分别为20.48、22.51、18.86、26.02和46.03、48.89、40.99、41.84，P均<0.05。 结论浙江地区男性HPV感染者主要以6、11型为主，高发于青壮年，HPV混合感染比例较高，高低危混合感染较常见。

Reverse dot hybridization analysis of epidemiological characteristics of 4620 HPV infected men in Zhejiang Province

ObjectiveTo investigate the subtype distributions and epidemiological characteristics of human papilloma virus (HPV) infection in Zhejiang Province and to provide evidence for early screening, prevention and diagnosis of male HPV infection. MethodsA retrospective analysis of 4620 male swabs or tissue specimens of genital warts from 17 to 74 years old in Zhejiang Province during January 2014 to December 2016 was performed. Of the 4620 samples, 120 samples were out of the study because of insufficient cell volume, the remaining 4500 samples were divided into 6 groups according to the ages (268 in the 17 to 20-year-old group, 1958 in the 21 to 30-year-old group, 1156 in the 31 to 40-year-old group, 667 in the 41 to 50-year-old group, 355 in the 51 to 60-year-old group and 96 in the 60-year-old group). Twenty-three HPV genotypes were determined by polymerase chain reaction (PCR) and reverse dot hybridization, then we analyzed the HPV positive rate distribution, HPV positive rate difference and high and low risk HPV infection characteristics. ResultsOf the 4500 samples, 2799 were positive for HPV with positive rate was 62.2%. Among them, type 6 and 11 HPV is the most prevalent, which account for 15.58% (436/2799) and 10.00% (280/2799) respectively. The most prevalent high-risk HPV type was type 52, accounting for 4.72% (132/2799). Of the 2799 HPV DNA-positive individuals, 1266 were single infections. Mixed infections occurred in 1533 cases, accounting for 44.26%. The highest single low-risk type was triple infection, the highest single high-risk type was quadruple infection, while the high-risk mixed infection reached seven, of which seven infections accounted for 2.25% (63/2799 cases).. The number of high-risk mixed infections was higher than that of high-risk and low-risk. Statistically significant difference was found between the two groups (χ²=28.1, and 82.8, respectively, P<0.05). HPV positive results were mainly found in young adults aged 21 to 40, accounting for 69.2% (3114/4500). The HPV positive rate was highest in the 17 to 20-year-old group (75.0%) and the 51 to 60-year-old group (79.7%). Compared with other age groups 21 to 30 years old (60.7%), 31 to 40 years old (59.1%), 41 In the 50-year-old (59.6%) and the >60-year-old (46.4%) groups, the χ² were 20.48, 22.51, 18.86, 26.02, and 46.03, 48.89, 40.99, and 41.84, respectively, P<0.05. ConclusionThe male HPV infections in Zhejiang Province are mainly type 6 and 11. The main types of male HPV infection in Zhejiang Province are type 6 and 11 and are more common in young and middle-aged people. The proportion of mixed HPV infection is high, and high and low risk mixed infection are more common.