腹泻型肠易激综合征患者肠黏膜肥大细胞的变化

目的探讨腹泻型肠易激综合征(IBS)与肠黏膜肥大细胞(MC)及脱颗粒之间的关系。方法正常人10例和腹泻型IBS患者11例。每例于结肠镜下取盲肠、横结肠和直肠黏膜组织各2块,用免疫组化方法行MC染色,计算每高倍视野下MC的数量及脱颗粒MC所占MC总数的比例。结果腹泻组IBS患者盲肠、横结肠黏膜MC数量显著高于正常对照组(P<0·01及P<0·05);直肠黏膜MC数量与正常对照组无显著差异(P>0·05)。腹泻型IBS患者盲肠、横结肠、直肠黏膜脱颗粒MC比率显著高于正常对照组(P<0·05或P<0·01)。结论肠黏膜肥大细胞在腹泻型IBS的发病过程中起着重要作用。

The Change of Mast Cells in Colon Mucosa of Patients with Diarrhea Irritable Bowel Syndrome

Objective To study alterations of mast cells in colon mucosa of patients with diarrhea irritable bowel syndrome(IBS).Methods Ten healthy volunteers and 11 diarrhea IBS entered in this study.By colonoscopy biopsies,the mast cells(MC)in mucosa of cecal colon,transverse colon and rectum were stained by immunohistochemistry and the number of mast cells and percentage of degranulated mast cells were counted.Results MC number increased significantly in the cecum and transverse colon of diarrhea IBS patients compared with controls(P<0.01 or P<0.05).The percentages of degranulated mast cells in cecal colon,transverse colon and retum mucosa of diarrhea IBS patients were significantly higher than controls(P<0.01 or P<0.05).Conclusion The mast cells play an important role in the pathogenesis of irritable bowel syndrome.