兔角膜碱烧伤新鲜羊膜移植后的角膜病理学及超微结构观察

目的观察兔角膜碱烧伤立即行新鲜羊膜移植后的角膜病理学和超微结构变化。方法40只新西兰白兔中随机取4只作为正常对照组,剩余36只制作双眼碱烧伤模型,右眼立即行新鲜羊膜移植术,左眼不做羊膜移植。分别在术后7、14、28d取材分别行HE染色和透射电镜观察。结果光镜、电镜结果显示:羊膜移植组与未移植组相比,上皮愈合好,基质纤维排列整齐,炎细胞浸润轻,新生血管少。结论兔角膜碱烧伤急性期行新鲜羊膜移植可以减少炎症反应、促进角膜上皮愈合、减轻角膜瘢痕形成、减少角膜新生血管形成。

Histopathological and ultrastructural observation of fresh amniotic membrane transplantation for corneal alkali burn

Objective To observe the changes of corneal histopathology and ultrastructure after fresh amniotic membrane transplantation in early treatment of corneal alkali burn. Methods 4 rabbits were chosen randomly as normal control from 40 normal New Zealand white rabbits.The models of corneal alkali burns were set up in the others. Amniotic membrane transplantation were performed on right eyes immediately after burn(group A), The left eyes were uncovered with amniotic membrane(group B). The rabbits were killed on the7th, 14th, 28th day, the corneas were removed for Histopathological and ultrastructural observation. Results In the aspects of corneal epithelium healing, the line up of stroma fiber, the infilitration of PMN, the corneal neovascularization, the group A was better than the group B. Conclusion In early treatment of corneal alkali burn, amniotic membrane transplantation was an effective method for reconstructing epithelium and restraining inflammation and scar and neovascularization.