|
|||||
|
|
| 非嵌合性抗癌药物队小牛胸腺DNA拓扑异构酶I活力的影响 | |
| 引用本文： | 张卫升,林卓坤,黄熙泰. 非嵌合性抗癌药物队小牛胸腺DNA拓扑异构酶I活力的影响[J]. 药学学报, 1990, 25(9): 641-645 |
| 作者姓名： | 张卫升 林卓坤 黄熙泰 |
| 作者单位： | 南开大学生物系,天津300071 |
| 摘 要： | 本文介绍从小牛胸腺中分离纯化DNA拓扑异构酶Ⅰ(简称拓扑酶Ⅰ)的方法,并用于检测了几种非嵌合性抗癌药物对该酶活力的影响。实验结果表明,一些已知的抗癌药物确有抑制DNA拓扑酶Ⅰ的作用。以抑制DNA拓扑酶Ⅰ为检测指标的方法可为筛选抗癌药物提供新的手段,并为药物抗癌机制的研究开辟了新的途径。 |
| 关 键 词： | 拓扑异构酶Ⅰ 抗癌药物 药物筛选 |
| 收稿时间： | 1989-07-31 |
Effect of some nonintercalative antitumor drugs on the activity of calf thymus DNA topoisomerase I |
|
| WS Zhang,ZK Lin and XT Huang. Effect of some nonintercalative antitumor drugs on the activity of calf thymus DNA topoisomerase I[J]. Acta pharmaceutica Sinica, 1990, 25(9): 641-645 | |
| Authors: | WS Zhang ZK Lin XT Huang |
| Affiliation: | Department of Biology, Nankai University, Tianjin. |
| Abstract: | DNA topoisomerase I has been isolated from the nuclei of calf thymus by PEG fractionation and chromatography on P11 and on Bio-Rex 70. Either a positive or negative supercoiled pBR322 DNA can be relaxed by the enzyme. The activity of Topo I is Mg2+ and ATP independent. Some of nonintercalative antitumor drugs such as camptothecine, hydroxycamptothecine, cyclophosphamide, methotrexate and mitomycin C were found to inhibit the activity of Topo I. The results suggest that DNA Topo I can be used to screen new nonintercalative antitumor drugs as a target protein. |
| Keywords: | Antitumor drugs Drugs screening Topoisomerase Ⅰ |
| 本文献已被 维普 等数据库收录！ | |
| 点击此处可从《药学学报》浏览原始摘要信息 | |
| 点击此处可从《药学学报》下载全文 | |