云南甘草中新三萜皂甙元的结构鉴定

从云南甘草(Glycyrrhiza yunnanensis)根中分离出10个化合物。本文报道其中二个新三萜皂甙元,云南甘草皂甙元A和B(glyyunnansapogenin A and B),以及已知化合物β-谷甾醇的鉴定。云南甘草皂甙元A和B的结构经衍生物制备,红外,核磁共振氢谱、碳谱和质谱等光谱测定和解析、分别推定为3β,24-dihydroxy-16-oxo-olean-12-en-29-oic acid和3β,21α,24-trihydroxy-olean-12-en-30-oic acid。

NEW TRITERPENOIDAL SAPOGENINS FROM THE ROOTS OF GLYCYRRHIZA YUNNANENSIS

Two new pentacyclic triterpenoidal sapogenins of oleanane type, namely glyyunnansapogenin A (I) and glyyunnansapogenin B (II), together with a known compound beta-sitosterol were isolated and characterized from the roots of Glycyrrhiza yunnanensis Cheng f. et L. K. Tai, family Leguminosae, collected in Yunnan Province, China. I was obtained as its methyl ester (Ia), mp 276-277 degrees C, which formed a diacetate (Ic), mp 244-245 degrees C. II, mp 287-289 degrees C, gave a triacetate (IIb), mp 286-287 degrees C and a monomethyl ester (IIa), mp 272-275 degrees C, which further formed a triacetate (IIc), mp 187-189 degrees C and a diacetate (IId), mp 243-245 degrees C after treatment with acetic anhydride and pyridine over night at room temperature. The structures of I and II were established by spectral analyses of IR, 1H NMR, 13CNMR, MS, CD and ORD. Glyyunnansapogenin A (I) was proved to be 3 beta, 24-dihydroxy-16-oxo-olean-12-en-29-oic acid and glyyunnansapogenin B (II) 3 beta, 21 alpha, 24-trihydroxy-olean-12-en-30-oic acid respectively.