| HPLC梯度洗脱法同时测定玉女煎多指标成分含量 |
| |
| 引用本文： | 王婷婷,王亚静,孙爱珍,周浩,朱光媚,李娜,张哲,田倩瑜.HPLC梯度洗脱法同时测定玉女煎多指标成分含量[J].天津中医药大学学报,2017,36(3):214-218. |
| |
| 作者姓名： | 王婷婷 王亚静 孙爱珍 周浩 朱光媚 李娜 张哲 田倩瑜 |
| |
| 作者单位： | 天津中医药大学, 现代中药发现与制剂技术教育部工程研究中心, 天津 300193,天津中医药大学, 现代中药发现与制剂技术教育部工程研究中心, 天津 300193,天津中医药大学, 现代中药发现与制剂技术教育部工程研究中心, 天津 300193,天津中医药大学, 现代中药发现与制剂技术教育部工程研究中心, 天津 300193,天津中医药大学, 现代中药发现与制剂技术教育部工程研究中心, 天津 300193,天津中医药大学, 现代中药发现与制剂技术教育部工程研究中心, 天津 300193,天津中医药大学, 现代中药发现与制剂技术教育部工程研究中心, 天津 300193,天津中医药大学, 现代中药发现与制剂技术教育部工程研究中心, 天津 300193 |
| |
| 基金项目： | 国家科技部“重大新药创制”专项课题（2013ZX09508-105） |
| |
| 摘 要： | 目的]建立同时测定玉女煎中芒果苷、β-蜕皮甾酮、毛蕊花糖苷含量的高效液相色谱法。方法]采用Ultimate XB-C_(18)色谱柱(4.6 mm×250 mm,5μm);以乙腈-0.1%磷酸溶液为流动相,梯度洗脱,流速:1.0 m L/min;检测波长:250 nm(芒果苷、β-蜕皮甾酮)和330 nm(毛蕊花糖苷)。结果]芒果苷、β-蜕皮甾酮、毛蕊花糖苷质量浓度在10.0~400.0μg/m L(r=0.999 9)、2.4~96μg/m L(r=0.999 9)、2.0~80μg/m L(r=0.999 9)范围内线性关系良好,3种成分的平均加样回收率(n=9)分别为100.2%、101.0%、98.1 8%,RSD分别为2.73%、1.95%、1.39%。结论]该方法简便,重复性好,可为玉女煎的质量控制提供参考。
|
| 关 键 词： | 玉女煎 梯度洗脱 同时测定 高效液相色谱法 芒果苷 β-蜕皮甾酮 毛蕊花糖苷 |
| 收稿时间： | 2017/3/5 0:00:00 |
HPLC for simultaneous determination of various quota components in Yunvjian |
| |
| WANG Ting-ting,WANG Ya-jing,SUN Ai-zhen,ZHOU Hao,ZHU Guang-mei,LI N,ZHANG Zhe and TIAN Qian-yu.HPLC for simultaneous determination of various quota components in Yunvjian[J].Journal of Tianjin University of Traditonal Chinese Medicine,2017,36(3):214-218. |
| |
| Authors: | WANG Ting-ting WANG Ya-jing SUN Ai-zhen ZHOU Hao ZHU Guang-mei LI N ZHANG Zhe and TIAN Qian-yu |
| |
| Institution: | Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China,Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China,Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China,Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China,Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China,Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China,Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China and Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China |
| |
| Abstract: | |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《天津中医药大学学报》浏览原始摘要信息 |
| 点击此处可从《天津中医药大学学报》下载免费的PDF全文 |
|
