血小板激活因子对大鼠脑损伤的实验研究

目的研究血小板激活因子(platelet-activatingfactor,PAF)在缺血性脑损伤中的致病作用。方法将PAF或含0.25%的牛血清白蛋白(BSA)生理盐水注入大鼠右侧颈总动脉,检测给药后即刻和40min的平均动脉压(MAP)和脑血流(CBF);用干湿重量法检测给药侧大鼠脑水含量变化;观察大鼠脑组织病理学改变。结果给药后即刻和40min对照组与PAF组MAP、CBF比较,两3者差异均显著(P<0.05)。两组脑组织水含量相比(P<0.01),差异也十分显著。对照组神经组织结构、毛细血管均未见异常改变。PAF组光镜下仅见小血管周围间隙扩张。电镜下皮层超微结构变化明显。结论PAF在诱发大鼠系统性低血压的同时降低脑血流,破坏血脑屏障,损伤神经细胞和其他神经组织。

Study on the injury effect of PAF on rat brain

Objective To study the pathogenic effect of platelet activating factor (PAF) on ischemic brain injury. Methods 36 Wister rats.PAF or 0.25% BSA saline was infused into the right carotid artery for 20min. MAP,CBF and the changes of brain water content (BWC) were measured.The pathologic changes of brain were observed under light and electron microscopy. Results MAP and CBF were significant difference between PAF with control groups (P< 0.05). BWC was significant difference between 2 groups (P< 0.05).Under light microscopy, only expanded fissures around small vascular were observed in PAF group. But ultrastructures were destroyed significantly. Conclusions PAF caused systemic hypotension, decreased CBF, destroyed blood brain barrier and damaged neurons and other neural tissues.