微囊化活性双歧联菌对失血性休克大鼠肠道屏障功能的保护作用

目的 观察给予双歧杆菌及其微囊化制剂预先处理对大鼠失血性休克模型肠道屏障和细菌移位的影响.方法 SD大鼠每天经口给予磷酸盐缓冲液(PBS)盐水、双歧杆菌(1×109cfu)或相同数量的微囊化双歧杆菌,喂养7 d后分别行90 min的休克或假休克,液体复苏后3 h剖腹取样行肠道菌群分析、细菌移位和回肠病理学检测.结果 3种不同处理的失血性休克大鼠组间总失血量相似.双歧杆菌预先处理可降低失血性休克大鼠盲肠内总需氧菌数量,微囊化双歧杆菌可降低更加明显.盲肠内总厌氧菌和双歧杆菌数量,双歧杆菌组增高,双歧杆菌微囊组更高.休克大鼠细菌移位到肠系膜淋巴结的发生率,双歧杆菌微囊组较PBS组降低;总需氧菌移位幅度,双歧杆菌休克大鼠的较PBS组降低,微囊组休克大鼠的细菌移位幅度较双歧杆菌组进一步降低;脾的总厌氧菌和双歧杆菌移位幅度却较PBS休克组显著性降低.双歧杆菌预先处理后休克大鼠的的绒毛损伤百分率,较PBS休克组明显减轻.结论 双歧杆菌可保护失血性休克大鼠的肠道屏障、减轻细菌移位,微囊化双歧杆菌可进一步增强这一效果.

Protective effects of micro-encapsulated Bifidobacteria on gut barrier after hemorrhagic shock and resuscitation: experiment with rats

Objective To investigate the effects of micro-encapsulated bifidobacteria on gut harrier and bacterial translocation after hemorrhagic shock and resuscitation. Methods Sprague-Dawley rats were divided into 6 groups : PBS + sham shock group fed with PBS for 7 days and then undergoing sham shock, bifidobacteria + sham shock group fed with bifidobacteria (109cfu/d) for 7 days and then undergoing sham shock, micro-encapsulated bifidobacteria + sham shock group, fed with micro-encapsulated bifidobacteria (109 cfu/d) for 7 days and then undergoing sham shock, PBS + hemorrhagic shock group fed with PBS for 7 days and then undergoing hemorrhagic shock, bifidobacteria + shock group fed with bifidobacteria for 7 days and then undergoing hemorrhagic shock, and micro-encapsulated bifidobacteria + shock group, fed with micro-encapsulated bifidobacteria for 7 days and then undergoing hemorrhagic shock. Three hours after resuscitation laparotomy was performed, distal cecum was resected to undergo bacteriological analysis of the cecal content, mesenteric lymph nodes (MLNs), a liver lobe, and the middle part of spleen were resected to undergo bacterial culture for bacterial translocation, and the terminal ileum was resected to observe the villous damage. Results There was no significant difference in the amount of blood loss among the 3 hemorrhagic shock groups. The amounts of aerobes in cecum of the bifidobactefia + shock and micro-encapsulated bifidobacteria + shock groups, especially that of the latter group, were significantly lower than that of the PBS + shock group. The amounts of anaerobes and the amounts of bifidobacteria in cecum of the bifidobacteria + shock group and micro-encapsulated bifidobacteria + shock group, especially those of the latter group, were significantly higher than those of the PBS + shock group. No bacterial translocation to liver was observed in all groups. The magnitudes of total aerobes translocation in spleen of the bifidobacteria +shock and encapsulated bifidobaeteria + shock groups were significantly lower than that of the PBS + shoc group, however, there were not significant differences in the translocation in the MEN of total aerobes ad bifidobacteria among different groups. The percentage of ileal villous damage of the bifidobacteria + shock and encapsulated bifidobacteria + shock groups were significantly lower than that of the PBS + shock group. Conclusion Bifidobacteria effectively protects the gut barrier, reduces bacterial translocation from the gut after hemorrhagic shock and resuscitation. And micro-encapsulated Bifidobacteria can enhance those effects further.