颅脑损伤患者细胞免疫功能变化的临床研究

目的：探讨颅脑损伤对患者细胞免疫功能的影响及其变化规律。方法：随机选取伤后２４小时内入院的颅脑损伤患者４８例（实验组），对照组选择同期住院的颅骨缺损修补术术前患者９例，实验组患者按格拉斯哥昏迷评分法（ＧＣＳ）评分及预后分组后，在不同时间分别采集血清及血涂片标本。采用放射免疫法、双抗体夹心酶联免疫吸附法（ＥＬＩＳＡ０及碱性磷酸酶法（ＡＰＡＡＰ０法分别检测患者血中白细胞介素－２（ＩＬ－２）、可溶性白细

Changes in cellular immunological function in patients with craniocerebral injury

Objective:To explore the changes in the cellular immunologic function in patients with cerebral injury.Methods:48 patients who were admitted to the hospital within 24 hours postinjury were randomly selected and 9 patients with skull defect were selected as the control.The patients were divided into groups in term of Glasgow coma score(GCS).Blood samples were collected accordingly and assayed using radioimmunoassay,sandwiched enzymelinked immunosorbent assay(ELISA) and alkaline phosphataseantialkaline phosphatase(APAAP) methods.Results:Blood interleukin2(IL2) concentration,T lymphocyte CD4 subpopulation and the ratio of CD4/CD8 in patients with craniocerebral injury decreased obviously within 24 hours while soluble interleukin2 receptor (SIL2R) and CD8 increased significantly.The lower the GCS,the more obvious were the changes in these parameters.The alteration was most obvious in nonsurvivors of severely injured group.These immunological indexes tended to return to normal level about fourteen days after injury.The indexes were of statistical significance among the patients with different outcome.Conclusions:Cellular immunity impairment exist in patients with head injury.The extent of cellular immunity suppression is correlated with the severity of the injury scaled by GCS.