| 利用双向电泳技术分析正常大鼠肾小球蛋白质组方法的建立 |
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| 引用本文： | 吕杨,谢院生,张术佳,李晓帆,谢振声,杨福全,陈香美.利用双向电泳技术分析正常大鼠肾小球蛋白质组方法的建立[J].中国中西医结合肾病杂志,2010,11(3):197-199. |
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| 作者姓名： | 吕杨 谢院生 张术佳 李晓帆 谢振声 杨福全 陈香美 |
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| 作者单位： | 1. 解放军总医院肾病科,全军肾脏病研究所暨重点实验室,北京,100853
2. 中国科学院生物物理研究所,北京,100101 |
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| 基金项目： | 国家自然科学基金重点资助项目(No.30630033) |
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| 摘 要： | 目的：利用双向电泳技术分析大鼠肾小球蛋白质,以建立肾小球蛋白质组分析的方法。方法：选取体重约200g的雄性Wistar大鼠,分离肾小球并提取蛋白质。使用24cm、pH3-11的IPG胶条对蛋白质进行一相等电聚焦,采用12.5%聚丙烯酰胺凝胶进行二相分离。Decyder软件分析凝胶上的蛋白质点,切取1个蛋白质点,经过胰蛋白酶消化后,使用飞行质谱获取肽指纹谱,并用Mascot软件分析。结果：纯化的肾小球纯度在90%以上,在双向电泳的凝胶上,共发现1240个肾小球蛋白质;切取的蛋白质点经鉴定为CAPZA2蛋白质,Mascot得分为137分（阈值为61分）。结论：成功建立了双向电泳分析大鼠肾小球蛋白质组的方法。
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| 关 键 词： | 双向电泳 肾小球 CAPZA2 |
Establishment of Method on Analyzing the Proteomics of Rat Glomeruli by Two-dimensional Gel Electrophoresis |
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| LV Yang,XIE Yuansheng,ZHANG Shujia,et alInstitute of Nephrology Key Laboratory of PLA,PLA General Hospital,Beijing.Establishment of Method on Analyzing the Proteomics of Rat Glomeruli by Two-dimensional Gel Electrophoresis[J].Chinese Journal of Integrated Traditional and Western Nephrology,2010,11(3):197-199. |
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| Authors: | LV Yang XIE Yuansheng ZHANG Shujia Institute of Nephrology Key Laboratory of PLA PLA General Hospital Beijing |
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| Abstract: | Objective:To establish the method of analyzing the proteomics of rat glomeruli by Two-dimensional gel electrophoresis (2-DE).Methods:The glomeruli protein isolated from 200 g wistar rat was separated by 24 cm pH 3-11 IPG strip and 12.5% polyacrylamide gel.The number of protein spot was calculated by Decyder software.The protein spot was digested by tripsin and the peptide mapping fingerprint was obtained by Maldi-tof.The results were analyzed by Mascot software.Results:The purity of glomeruli was more than ... |
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| Keywords: | 2-D Glomeruli CAPZA2 |
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