| 雷公藤内酯醇下调P53基因甲基化抑制肝癌SMMC-7721细胞的增殖 |
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| 引用本文： | 吴瑛,刘芳,郭文渊,肖亮,阳揭宇,谢江平,丁国善.雷公藤内酯醇下调P53基因甲基化抑制肝癌SMMC-7721细胞的增殖[J].中国肿瘤生物治疗杂志,2011,18(3). |
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| 作者姓名： | 吴瑛 刘芳 郭文渊 肖亮 阳揭宇 谢江平 丁国善 |
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| 作者单位： | 1. 解放军第85医院药剂科,上海,200235
2. 第二军医大学长征医院器官移植科,上海,200003 |
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| 基金项目： | 国家自然科学基金资助项目(No.81072444)~~ |
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| 摘 要： | 目的:研究雷公藤内酯醇(triptolide,TP)对人肝癌SMMC-7721细胞增殖的影响以及对P53基因的去甲基化作用。方法:MTT法检测TP对SMMC-7721细胞增殖的影响,甲基特异性PCR检测TP对SMMC-7721细胞P53基因甲基化的影响,RT-PCR检测SMMC-7721细胞甲基转移酶DNMT1、DNMT3a、DNMT3bmRNA的表达,Western blotting检测SMMC-7721细胞中P53蛋白的表达。结果:TP剂量依赖性抑制SMMC-7721细胞的增殖(P<0.05),40 ng/ml时的抑制率达(73.5±3.02)%,其半数抑制浓度(IC50)约为20 ng/ml。TP显著抑制SMMC-7721细胞中DNMT1、DNMT3a、DNMT3bmRNA的表达(P<0.05,P<0.01);TP作用后P53基因的高甲基化被逆转,并呈剂量依赖性;TP可显著增强SMMC-7721细胞中P53蛋白的表达。结论:TP可通过抑制甲基转移酶使P53基因去甲基化,促进P53蛋白的表达,从而抑制SMMC-7721细胞的增殖。
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| 关 键 词： | 雷公藤内酯醇 P53基因 甲基化 SMMC-7721细胞 甲基转移酶 |
Triptolide down-regulates P53 gene methylation and inhibits proliferation of hepatocarcinoma SMMC-7721 cells |
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| WU Ying,LIU Fang,GUO Wen-yuan,XIAO Liang,YANG Jie-yu,XIE Jiang-ping,DING Guo-shan.Triptolide down-regulates P53 gene methylation and inhibits proliferation of hepatocarcinoma SMMC-7721 cells[J].Chinese Journal of Cancer Biotherapy,2011,18(3). |
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| Authors: | WU Ying LIU Fang GUO Wen-yuan XIAO Liang YANG Jie-yu XIE Jiang-ping DING Guo-shan |
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| Institution: | WU Ying1,LIU Fang2,GUO Wen-yuan2,XIAO Liang2,YANG Jie-yu2,XIE Jiang-ping2,DING Guo-shan2(1.Department of Pharmacy,No.85 Hospital of PLA,Shanghai 200235,2.Organ Transplantation Center,Changzhang Hospital,Second Military Medical University,Shanghai 200003,China) |
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| Abstract: | Objective: To study the effect of triptolide(TP) on the proliferation of hepatocarcinoma SMMC-7721 cells and its effect on demethylation of P53 gene.Methods: The effect of TP on proliferation of SMMC-7721 cells was measured by MTT method,the effect of TP on P53 gene methylation in SMMC-7721 cells was analyzed by methylation specific PCR,the expressions of methyltransferase DNMT1,DNMT3a and DNMT3b mRNA in SMMC-7721 cells were measured by RT-PCR,and the protein expression of P53 in SMMC-7721 cells was detecte... |
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| Keywords: | triptolide P53 gene methylation SMMC-7721 cell methyltransferase |
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