| mda7-/IL2-4和IL2-4delE5诱导急性髓系白血病细胞向单核细胞分化 |
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| 引用本文： | 姚兴荣,马小彤,杨宾霞,段永娟,林永敏.mda7-/IL2-4和IL2-4delE5诱导急性髓系白血病细胞向单核细胞分化[J].中国肿瘤生物治疗杂志,2011,18(3). |
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| 作者姓名： | 姚兴荣 马小彤 杨宾霞 段永娟 林永敏 |
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| 作者单位： | 中国医学科学院北京协和医学院血液病医院暨血液学研究所实验血液学国家重点实验室,天津,300020 |
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| 基金项目： | 国家自然科学基金资助项目(No.30872983,81070426); 天津市应用基础研究基金重点资助项目(No.11JCZDJC18000)~~ |
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| 摘 要： | 目的:研究人黑素瘤分化相关基因-7(melanoma differentiation associated gene-7,mda-7;又称IL-24)及其剪接体IL-24 delE5与急性髓系白血病(acute myeloid leukemia,AML)细胞分化的关系。方法:十四烷酰佛波醇-乙酯(12-O-tetrade-canoylphorbol-13-acetate,TPA)处理人急性髓系白血病细胞系U937、HL-60,real-time PCR及Western blotting检测细胞中mda-7/IL-24和IL-24 delE5的表达,FACS检测细胞表面CD11b、CD14和CD115的表达。siRNA干扰U937、HL-60细胞中mda-7/IL-24和IL-24 delE5的表达后,再以TPA诱导细胞分化,FACS检测CD11b、CD14和CD115的表达。用前期构建的mda-7/IL-24和IL-24 delE5载体转染U937、HL-60及AML-M5患者的原代白血病细胞,观察异位过表达mda-7/IL-24和IL-24 delE5能否诱导白血病细胞向单核细胞分化。结果:TPA在诱导U9...
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| 关 键 词： | 黑素瘤分化相关基因-7(mda-7/IL-24) IL-24delE5 十四烷酰佛波醇-乙酯 急性髓系白血病 单核细胞 分化 |
mda-7/IL-24 and IL-24 delE5 induce myeloid leukemia cells to differentiate into monocytes |
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| YAO Xing-rong,MA Xiao-tong,YANG Bin-xia,DUAN Yong-juan,LIN Yong-min.mda-7/IL-24 and IL-24 delE5 induce myeloid leukemia cells to differentiate into monocytes[J].Chinese Journal of Cancer Biotherapy,2011,18(3). |
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| Authors: | YAO Xing-rong MA Xiao-tong YANG Bin-xia DUAN Yong-juan LIN Yong-min |
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| Institution: | YAO Xing-rong,MA Xiao-tong,YANG Bin-xia,DUAN Yong-juan,LIN Yong-min(State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Diseases Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300020,China) |
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| Abstract: | Objective:To investigate the effects of melanoma differentiation associated gene-7(mda-7,also named IL-24) and IL-24 delE5,an mda-7/IL-24 splice variant,on differentiation of acute myeloid leukemia(AML) cells.Methods: The AML cell lines U937 and HL-60 were treated with 12-O-tetradecanoylphorbol-13-acetate(TPA);mda-7/IL-24 and IL-24 delE5 expressions were detected by real-time PCR and Western blotting assays.CD11b,CD14 and CD115 expressions on cell surface were examined by FACS.U937 and HL-60 cells were indu... |
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| Keywords: | melanoma differentiation associated gene-7(mda-7/IL-24) IL-24 delE5 12-O-tetradecanoylphorbol-13-acetate(TPA) acute myeloid leukemia monocyte differentiation |
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