| miR-199a-3p对前列腺癌细胞迁移及侵袭能力的影响 |
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| 引用本文： | 孔繁飞,王中显,孙朝阳,吕煊,翁丹卉,卢运萍,陈刚,吴明富. miR-199a-3p对前列腺癌细胞迁移及侵袭能力的影响[J]. 肿瘤防治研究, 2011, 38(8): 875-877. DOI: 10.3971/j.issn.1000-8578.2011.08.006 |
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| 作者姓名： | 孔繁飞 王中显 孙朝阳 吕煊 翁丹卉 卢运萍 陈刚 吴明富 |
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| 作者单位： | 430030武汉，华中科技大学同济医学院附属同济医院妇产科 肿瘤生物医学中心 |
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| 基金项目： | 国家科技部973计划基金资助项目(2009CB521808); 教育部新教师基金资助项目(200804871056); 湖北省卫生厅青年科技人才基金资助项目(QJX2010-3) |
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| 摘 要： | 目的从miR-199a-3p在前列腺癌高、低转移潜能细胞中的表达差异出发，研究其在高转移前列腺癌细胞株1E8细胞运动转移中的作用及可能的分子机制。方法运用Realtime PCR法检测 miR-199a-3p在前列腺癌高、低转移潜能配对细胞系中的表达差异。通过划痕实验及transwell实验观察1E8细胞及转染 miR-199a -3p mimics及mimics NC后该细胞运动迁移能力的变化。结果（1）Realtime PCR结果显示高转移潜能1E8细胞中miR-199a-3p表达水平明显低于低转移潜能2B4细胞。（2）上调miR-199a-3p会减弱1E8细胞的运动转移能力。结论miR-199a-3p的上调可以抑制前列腺癌细胞的运动迁移能力。
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| 关 键 词： | 前列腺癌 miR -199a-3p microRNA |
| 收稿时间： | 2011-02-11; |
Effects of miR-199a-3p on Cell Migration and Invasion in Prostate Cancer Cells |
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| KONG Fan-fei,WANG Zhong-xian,SUN Chao-yang,LV Xuan,WENG Dan-hui,LU Yun-ping,CHEN Gang,WU Ming-fu. Effects of miR-199a-3p on Cell Migration and Invasion in Prostate Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2011, 38(8): 875-877. DOI: 10.3971/j.issn.1000-8578.2011.08.006 |
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| Authors: | KONG Fan-fei WANG Zhong-xian SUN Chao-yang LV Xuan WENG Dan-hui LU Yun-ping CHEN Gang WU Ming-fu |
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| Affiliation: | Department of Gymecology and ObstetricsCancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | Objective To investigate the different expression of miR-199a-3p in different prostate cancer cell lines and the effects of miR-199a-3p on cell migration and invasion in prostate cancer.Methods The expression of miR-199a-3p in 1E8(high metastasis prostate cancer) and 2B4(low metastasis prostate cancer) cell lines were identified by realtime PCR.The migration and invasion abilities were detected by Transwell experiment.Results(1)By comparing with 2B4 cells,of miR-199a-3p was decreased in 1E8 cells.(2)The inv... |
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| Keywords: | Prostate cancer miR-199a-3p microRNA |
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