| 银杏愈伤组织和悬浮细胞培养及黄酮类化合物的产生 |
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| 引用本文： | 张辉,于荣敏,姚新生. 银杏愈伤组织和悬浮细胞培养及黄酮类化合物的产生[J]. 沈阳药科大学学报, 1999, 16(2): 129-133 |
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| 作者姓名： | 张辉 于荣敏 姚新生 |
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| 作者单位： | 1. 沈阳药科大学制药系,沈阳,110015
2. 沈阳药科大学中药系,沈阳,110015 |
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| 摘 要： | 进行了银杏愈伤组织和悬浮细胞培养及其次生代谢产物———黄酮类化合物的研究．考察了不同理化因子对各种银杏外植体黄酮含量的影响．对银杏细胞培养物中的芦丁、槲皮素和山奈酚进行了ＨＰＬＣ的定量测定
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| 关 键 词： | 银杏；愈伤组织培养；细胞悬浮培养；黄酮类化合物；高效液相色谱 |
| 修稿时间： | :1998-06-2 |
Studies on the Callus Culture and the Suspension Culture of Ginkgo biloba and the Formation of Flavonoids |
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| Zhang Hui,Yu Rongmin,Yao Xinsheng. Studies on the Callus Culture and the Suspension Culture of Ginkgo biloba and the Formation of Flavonoids[J]. Journal of Shenyang Pharmaceutical University, 1999, 16(2): 129-133 |
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| Authors: | Zhang Hui Yu Rongmin Yao Xinsheng |
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| Abstract: | This paper reported firstly the callusculture & the suspension culture of Ginkgo biloba and the formation of its secondarymetabolites-flavonoids.The effects of some physico-chemical factors on theinduction,culture of callus and cells and the yield of flavonoids were alsoinvestigated.Finally,the quantitative determination by HPLC for rutin,quercetin andkaempferol in the cultures were showed in this study. |
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| Keywords: | Ginkgo biloba callus culture cell suspension culture flavonoids HPLC |
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