反相离子对HPLC法同时测定复方苯甲酸软膏中两组分的含量

目的:建立HPLC法同时测定复方苯甲酸软膏中苯甲酸和水杨酸含量的方法.方法:使用ZORBAX Eclipse XDB-C18(150 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-水(78∶22)含十六烷基三甲基溴化铵(HDTA)0.001 5 mol·L-1,用稀醋酸调pH至5],柱温为30℃,流速为1.0 ml· min-1,检测波长为280 nm.结果:苯甲酸和水杨酸的线性范围分别为:49.5 ～495.2 μg· ml-1 (r=1.000),24.3 ～243.2 μg·ml-1 (r=1.000)；平均回收率分别为99.8％,99.9％；RSD分别为0.99％,0.87％.结论:该方法快速,简便,灵敏,分离度好,适用于控制产品质量.

Determination of Benzoic Acid and Salicylic Acid in Compound Benzoic Acid Ointments by Ion-pair RP-HPLC

Objective： To establish an ion-pair RP-HPLC method for the determination of benzoic acid and salicylic acid in com- pound benzoic acid ointments. Method： A ZORBAX Eclipse XDB-C18 column （150 mm x 4. 6 mm,5 um） was used. The mobile phase was methanol-water （78： 22, including HDTA 0. 001 5 mol L-1, adjusting pH to 5.0 with dilute acetic acid） and the column tempera- ture was30 ℃. The flow rate was 1.0 mi.min-1 and the detection wavelength was 280 nm. Result： The linear range of benzoic acid and salicylic acid was 49. 5-495. 2 Ixg·m1-1 （r= 1. 000） and 24. 3-243.2 ug.ml-1 （r= 1. 000） with average recovery of 99. 8% （RSD =0. 99% ） and 99. 9% （RSD = 0. 87% ）, respectively. Conclusion： The method is simple, sensitive, accurate and suitable to control the preparation quality.