高效液相色谱法测定草乌类药用植物活性成分含量

 目的采用高效液相色谱测定草乌类药用植物中中乌头碱、乌头碱、次乌头碱的含量,对草乌类植物进行质量与资源评价。方法建立高效液相色谱方法,采用Phenomenex ODS色谱柱(4.6mm×250mm,5μm),以乙腈-醋酸铵缓冲溶液(pH10.5)(54∶46)为流动相,流速为1.0mL·min^-1;检测波长为240nm。结果中乌头碱在0.28～2.80μg内呈良好的线性关系(r=0.9991),平均回收率为98.9%(n=5),RSD=0.93%;乌头碱在0.136～1.36μg内呈良好的线性关系(r=0.9992),平均回收率为97.4%(n=5),RSD=1.18%;次乌头碱在0.248～2.48μg内呈良好的线性关系(r=0.9991),平均回收率为98.4%(n=5),RSD=1.11%。北乌头、乌头与小白掌含有中乌头碱、乌头碱、次乌头碱3种生物碱;多根乌头含有中乌头碱、乌头碱2种生物碱;其他的样品含微量或几乎没有。多根乌头的3种生物碱总量最高。结论本方法分离效果好,分析速度快,方法稳定,结果准确。

Determination of Active Ingredients in Radix Aconiti Kusnezoffii by HPLC

OBJECTIVE To determine the contents of mesaconitine(MA), aconitine(A) and hypaconitine(HA) in Radix Aconiti Kusnezoffii species by HPLC.METHODS A HPLC method was established with a Phenomenex ODS column (4.6 mm×250 mm,5 μm).The mobile phase consisted of acetonitrile-acetic acid ammonium buffer(pH 10.5) (54∶46)at a flow rate of 1.0 mL·min^-1.The detection wavelength was at 240 nm.RESULTS MA was linear within the range of 0.28～2.80 μg(r=0.999 1),the average recovery was 98.9%(n=5) and RSD was 0.93%.A was linear within the range of 0.136～1.36 μg(r=0.999 2),the average recovery was 97.4%(n=5) and RSD was 1.18%.HA was linear within the range of 0.248～2.48 μg(r=0.999 1),the average recovery was 98.4%(n=5) and RSD was 1.11%. Aconitum kusnezoffii Reichb, A.carmichaeli Debx and A. nagawm starf var.heterotuchum Fletcher et lauener contain MA, A and HA;Aconitum karakolicum Rap contained MA and A; others samples contained minimum or no target constitute.The contents of three alkaloids of A.karakolicum Rap was highest in all samples. CONCLUSION This method is easy, rapid, accurate and sensitive.