原位杂交法检测肝组织中输血传播病毒

采用对称和不对称PCR法 ,以地高辛素标记双链和单链探针 ,用原位杂交法检测了 56例肝组织中输血传播病毒 (TTV)核酸 ,以探讨TTV的致病性。发现 51例非甲～非庚型肝炎肝组织中TTV总检出率为 2 7.5% ( 1 4 /51 ) ;5例非肝炎肝组织杂交阴性。TTVDNA主要定位于肝细胞核内 ,受染肝细胞见于急性、慢性和重症肝炎的肝组织。双链探针杂交结果与单链探针检测病毒基因链的结果一致 ,2例 ( 2 /6)例组织TTV基因链与互补链同时存在。提示TTV是导致肝炎的一种新病原 ,并且在肝脏内有复制

Study on Transfusion Transmitted Virus in Liver Tissue by in Situ Hybridization

To study transfusion transmitted virus(TTV) infection from liver tissues, double strand and single strand probes were labelled with digoxigenin to detect 56 liver samples by in situ hybridization and by symmetrical and unsymmetrical polymerase chain reaction (PCR). TTV DNA was found in 14 of 51 patients with non hepatitis A and non hepatitis G (27.5%). Five liver samples without hepatitis were negative. TTV DNA was mainly observed in nuclei of hepatocytes and distributed in the tissues with acute , chronic and severe types of hepatitis. The hybridization result by double strand probe was consistent with TTV genome strand hybridization by single strand probe. In 2 of 6 liver samples were present both TTV genome strand and complementary strand. This study indicates that TTV is a novel virus which results in hapetitis and the virus replicates in liver tissue of the patients.