Evaluation of Some Pharmacological Activities of ethanol extracts of seeds, pericarp and leaves of Eugenia Jamolana in Rats |
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Authors: | S M A El-Shenawy |
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Institution: | (1) Dep. of Pharmacology, National Research Centre, Tahrir St., Dokki, Egypt |
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Abstract: | The present study investigated the effect of ethanol extracts of seeds, pericarp and leaves of Eugenia Jamolana (E. Jamolana) on inflammation, gastric ulcer, anti-oxidants and hepatoprotective in rats. The acute inflammation was induced by intra-plantar
injection of carrageenan (100 μl of 1 %) in the rat hind paw. Gastric ulcer was evoked by indomethacin (25 mg/kg) oral administration.
Liver damage was induced by given CCL4 (2.5 ml/kg) orally. The median lethal (LD50) of the ethanol extract of both seeds and pericarp were determined and revealed that the investigated extracts of seeds and
pericarp were non toxic up to 5g/kg. The anti-inflammatory results showed that the oral administration of ethanol extract
of E. Jamolana seeds (250,500 mg/kg) showed significant inhibition of oedema formation in dose-dependent manner by −27.86, −41.23, −44.73,
−51.78 % and by −63.16, −37.77, −47.04, −55.36 % at 1, 2, 3 and 4 h at 1, 2, 3 and 4 h, respectively. While the pericarp given
at dose (500 mg/kg) exhibited significant inhibition of the oedema formation by −34.64, −21,8, 19.23 and −33.47 % at 1, 2,
3 and 4 h, respectively post carrageenan injection as compared with saline control group. E. Jamolana leaves fraction 1 given orally at dose of 25 mg/kg, induced non significant change on oedema, while the oedema response was significantly inhibited
by −25.14, −33.4, −20.57 and −26.46 % at 1, 2, 3 and 4 h, respectively in group of rats that received leaves fraction 2 at the same dose. Rats were given leaves fraction 3 extract showed inhibition of oedema formation by −4.48 % at 1st h post- carrageenan injection, while at 2nd, 3rd and 4th h showed non significant change on oedema formation. The acute gastric mucosal lesions was significantly reduced by given
ethanol extract of E. Jamolana seeds, pericarp (250, 500 mg/kg) and leaves fractions 1, 2 and 3 (25 mg/kg) respectively in dose dependent manner, as compared
with indomethacin treated group (control group). All tested extracts showed significant reduction in elevated serum ALT, AST
and ALP levels as compared with CCl 4 treated group. The ethanol extract of E. Jamolana seeds, pericarp and leaves fractions 1, 2, 3 showed significant elevation of blood GSH level and significant reduction in
elevated plasma lipid peroxides (MDA) as compared with CCl4 treated group. In conclusion we can see that the ethanol extracts of E. Jamolana of seeds, pericarp and leaves fractions showed anti-inflammatory, anti-ulcer, hepatoprotective and anti-oxidants activity.
Received 15 June 2008; accepted 11 November 2008 |
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Keywords: | :" target="_blank">: E Jamolana Seeds Pericarp Leaves Inflammation Ulcer Liver enzymes CCL4 GSH MDA Rats |
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