成年大鼠嗅球成鞘细胞的原代培养

本实验取材于成年 Wistar大鼠嗅球的最外两层 ,进行原代培养嗅球成鞘细胞 ,用胶质纤维酸性蛋白抗体免疫组化方法确认。结果显示 :( 1)培养至第 10 d,原代培养物多见两种形态的细胞 :一种为单极、双极或多极细胞 ,带有细长的突起 ;另一种为所谓“煎蛋样”细胞 ,扁平状 ,胞质少极化 ,边缘不规则 ;( 2 )胶质纤维酸性蛋白反应显示这两种细胞均呈阳性 ,Nissl法复染后 ,可计数其纯度为 70 %～ 85 %。

PRIMARY CULTURE OF OLFACTORY ENSHEATHING CELLS FROM ADULT RAT OLFACTORY BULBS

Derived from adult rat olfactory nerve and glomerular layers of the olfactory bulb, olfactory ensheathing cells(OECs) could be cultured in vitro. We identified OECs with GFAP and estimated the purity of OECs in primary cultures according to immunohistochemistry and characteristics of morphology. The results were as follows: (1) At 10 th day in vitro, two different kinds of cells could be identified: one was the fusiform and multipolar cells with thin and long processes; the other was flat cells with fried egg-like morphology. (2) Both kinds of cells could be stained positively for glial fibrillary acidic protein(GFAP). After Nissl restaining, the purity of OECs was estimated to be about 70%～85%.