脂多糖对人工关节磨屑诱导单核细胞分泌功能的影响

目的探讨脂多糖（LPS）促进人工关节松动的可能性。方法采用酶联免疫吸附法（ELISA）测定在不同剂量LPS作用下超高分子聚乙烯微粒（UHMWPE）诱导人外周血单核细胞（MOs），培养3，6，12，24，48h肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）的含量变化。结果与空白对照组A组相比LPS组、UHMWPE组、及不同浓度的LPS联合UHMWPE刺激组刺激3h后TNF—α、IL-6显著升高（均P〈0．05），12h达高峰，TNF-α、IL-6分泌与LPS呈现浓度依赖性，刺激12，24，48hF组TNF—α、IL-6分泌明显高于A、B、C、D、E组（P〈0．05）。结论LPS能有效地促进由于UHMWPE刺激MOs所致的TNF—α、IL-6的分泌，增强了人工关节置换术后由微粒诱导的骨溶解。

LIPOPOLYSACCHARIDE ENHANCE THE SECRETORY FUNCTION OF HUMAN MONOCYTES INDUCED BY WEAR PARTICLES IN VITRO

Objective To investigate the possibility of Lipopolysaccharide （LPS） contribute to aseptic loosening of orthopedic implants. Methods Peripheral blood samples were obtained from healthy human donors. Monocytes （MOs） were isolated and assigned to 6 groups. Group A was treated with MOs alone, group B with MOs ＋ LPS （100ng/ ml）, group C with MOs ＋ ultrahigh molecular weight polylethylene （UHMWPE）, group D with MOs ＋ UHMWPE ＋ LPS （1ng/ml）, group E with MOs ＋ UHMWPE ＋ LPS 10ng/ml）, group F with MOs ＋ UHMWPE ＋ LPS 100ng/ml）. The contents of TNF-α and IL -6 in the culture supernatant were measured by method of enzyme linked immunoadsorbent assay （ELISA）. Results The released contents of TNF-α, IL- 6 from MOs were increased significantly compared with those before LPS or UHMWPE challenge（P〈0.05）. And the content of TNF-α, IL- 6 were gradually increased by LPS with different concentrations（P（0.05）. The content of targets above was little by little increased with stimulated time prolonging, then the peak of TNF-α, IL - 6 were appeared in 12h.Conclusions The inflammatory cytokine of TNF-α, IL- 6 are increased in the MOs stimulated by LPS. LPS and UHMWPE cooperative interact to enhance the biological effects of aseptic loosening.