非小细胞肺癌MDR1—mRNA,MRP—mRNA及LRP—mRNA表达的研究

目的：观察肺癌组织及癌旁肺组织MDR1－mRNA,MRP-mRNA及LRP－mRNA的表达,方法：采用RT－PCR法检测30例肺癌患者组织和正常肺组织中MDR1－mRNA,MRP-mRNA和LRP－mRNA的表达情况。结果：MDR1－mRNA在肺癌组织及癌旁肺组织的表达阳性率分别为40％及16．67％（P＝0．045）,其表面与细胞分化程度,发期及病理类型无明显关系,MRP－mRNA在肺癌组织及癌旁肺组织的表达分别为43＝．44％及26．67％,低分化者MRP的表达率明显高于中高分化者（P＝0．0004）,其表达与肿瘤类型,临床分期及癌细胞的分化程度无明显关系。30例肺癌组织中MDR1－mRNA,MRP-mRNA,LRP-mRNA共同表达者7例（23．33％）,三者均无表达者10例（33．33％）,三者的一致性达56．67％,结论：MDR1－mRNA,MRP-mRNA,LRP-mRNA在肺癌的耐药中可能起重要作用。

Expression of MDR1-mRNA, MRP-mRNA and LRP-mRNA in patients with non-small cell lung cancer

Objective To investigate the expression of multidrug resistance (MDR1), multidrug resistance associated protein (MRP) and lung resistance protein (LRP) genes in patients with non small cell lung cancer (NSCLC). Methods Expression of MDR1, MRP and LRP genes was detected in 30 NSCLC patients by RT PCR method. Results The positive rates of MDR1 expression were 40% and 16.67% respectively in lung cancer tissues and normal lung tissues (P=0.045), and it was not associated with the degree of cell differentiation, histological classification and the clinical stage. The positive rates of MRP expression were 43.33% and 26.67% respectively in lung cancer tissues and normal lung tissues. Its expression was related to degree of cell differentiation (P=0.03), but not to the histological classification and the clinical stage. LRP expression of lung cancer tissues (56.67%) was much higher than that of normal tissues (P= 0.000?4) , and it was not associated with degree of cell differentiation, histological classification and the clinical stage. Of the 30 lung cancer specimens, 7 expressed all the three kinds of genes, and 10 expressed none of them. The coincident rate was 56.67% . Conclusion The results suggest that MDR1, MRP and LRP gene may play important roles in drug resistance in NSCLC.