红花显微特征常数与化学成分相关性

目的:研究红花花粉粒显微特征常数的测定方法并测定红花中指标性成分羟基红花黄色素A的含量,分析两者的相关性,建立评价红花质量的新方法。方法:采用容量分析法对红花粉末中花粉粒的显微特征常数值进行测定,采用高效液相法对红花中羟基红花黄色素A的含量进行测定,将两组数据用统计方法进行相关性分析。结果:红花中花粉粒的显微特征常数与羟基红花黄色素A具有显著的相关性(正相关)。即红花花粉粒的显微特征常数的数值愈大,羟基红花黄色素A的含量愈大。结论:利用显微定量法测定红花显微特征常数值来评价其质量是科学、可行的。该研究初步建立了一种基于花粉粒显微特征常数测定的中药材质量评价新方法及技术,可为红花的质量控制提供新的技术手段,为其质标准制定提供参考依据。

Correlation Between Microscopic Characteristic Constants and Chemical Components of Carthami Flos

Objective: The aim of this article was to study onthe determination method of microscopic characteristic constants of pollen grains in Carthami Flos and detect the content of hydroxysafflor yellow A, and analysis of the correlation between the two sideswere analyzed. Method: Capacity analysis was used to quantify pollen grains of Carthami Flos and the content of hydroxysafflor yellow A was determined by HPLC. The data were analyzed by SPSS statistics software. Result: The microscopic characteristic constants of pollen grains and the content of hydroxysafflor yellow A in Carthami Flos were significantly correlated (positive correlation). In other words, results indicated that herbal samples with more pollen grains would contain greater amounts of the pharmacologically active components in Carthami Flos, thus be of better quality in clinical application. Conclusion: It is scientific and feasible to determine the quality of Carthami Flos is by the microscopic quantitative method. The method of based on the microscopic characteristic constants of pollen grains has been preliminarily established. It can be applied to the quality control of Carthami Flos. This approach can be further applied to other medicinal herbs, extending the use of microscopic techniques to the field of quality control of medicinal materials.