陈皮提取物改善慢性温和不可预知应激小鼠行为和海马BDNF的研究

目的:探讨陈皮提取物对慢性温和不可预知应激(CUMS)抑郁模型小鼠的行为和对脑源性神经营养因子(BDNF)表达水平的影响。方法:ICR小鼠根据糖水偏好百分比随机分为空白对照组、模型对照组、阳性对照盐酸氟西汀20 mg·kg-1组及陈皮提取物100,200,400 mg·kg-1组;除空白对照组外,各组均接受CUMS,造模28 d后开始灌胃给药;各组连续给药28 d后,糖水偏好实验测定糖水偏好百分比、新奇抑制摄食实验测定摄食潜伏期、强迫游泳实验测定不动时间;荧光实时定量PCR和ELISA法测定大脑海马BDNF含量。结果:慢性应激后,模型对照组小鼠糖水偏好百分比减少,摄食潜伏期延长,不动时间增多,海马BDNF含量显著降低;与模型对照组比较,陈皮提取物组能显著改善抑郁小鼠行为学指标,显著提高小鼠海马BDNF含量。结论:陈皮提取物对CUMS模型小鼠有抗抑郁作用,其机制可能与增加海马BDNF有关。

Extracts from Pericarpium citri Improve Behaviors and Hippocampal BDNF in Mice Exposed to Chronic Mild Unpredictable Stress

Objective: To investigate the antidepressant-like activity of extracts from Pericarpium citri, and its mechanism related to brain derived neurotrophic factor (BDNF) in mice exposed to chronic mild unpredictable stress (CUMS). Method: Male ICR mice were randomly divided into 6 groups:control group, CUMS group(20 mg·kg^-1), fluoxetine group and P. citri groups (100, 200, 400 mg·kg^-1). The treatment groups, except control group, were exposed to CUMS for 8 weeks and administrated for 4 weeks. Sucrose preference test, novelty suppressed feeding test and forced swimming test were used to evaluate the antidepressant-like effect of extracts from P. citri. BDNF expressions were measured by qPCR and ELISA. Result: CUMS induced reduction of sucrose preference, increase of latency to feed and immobility time, decrease of hippocampal BDNF expressions. Compared with CUMS mice, extracts of P. citri significantly increased sucrose preference, decreased latency to feed and immobility time. In addition, P. citri elevated the expression of BDNF in hippocampus. Conclusion: Our results suggest that extracts from P. citri exhibit antidepressant-like activity, which may be mediated by elevation of hippocampal BDNF.