抗核抗体荧光模式及滴度与免疫球蛋白及补体的关系分析

目的探讨抗核抗体荧光模式及滴度与免疫球蛋白及补体的关系。方法采用透射比浊法对768例抗核抗体阳性患者血清进行免疫球蛋白及补体检测,另选取200名健康体检者作为对照组。根据抗核抗体荧光模式及滴度进行分组,分别研究各组免疫球蛋白及补体水平。结果①抗核抗体滴度≥1∶100时,血清IgG、IgA明显升高(P<0.01),但升高的幅度与抗核抗体滴度无明显相关;IgM和C3、C4只有在抗核抗体滴度≥1∶1 000时才分别显著升高和下降(P<0.01)。②各种单荧光模式型患者血清IgG、IgA、IgM、C3、C4浓度无显著差异;与各单荧光模式患者比较,二联及二联以上荧光模式型患者血清IgG、IgA、IgM显著升高、补体C3、C4水平显著下降(P<0.01)。结论抗核抗体、免疫球蛋白及补体虽然与自身免疫性疾病高度相关,但并不与病情成平行关系,三项联合检测才能对疾病的诊断与病情发展作出正确判断。

Analysis of the relationship of human immunoglobulin and complement with fluorescent pattern and titer of antinuclear antibody

Objective To explore the relationship of human immunoglobulin and complement with fluorescent pattern and titer of antinuclear antibody.Methods The serum level of immunoglobulin and complement were detected by immuno-turbidimetric method in 768 sera of patients with positive antinuclear antibody.Two hundred normal healthy case were considered the control group.According to different patterns and titers,several groups were divided to investigate the change of immunoglobulin and complement.Results ①When the titer of antinuclear antibody was more than 1∶ 100,the level of IgG and IgA in patients,serum were obviously higher than those of normal control group（P 0.01）.But the increase of IgG and IgA was no significant correlation with antinuclear antibody titers.When the titer of antinuclear antibody was more than 1 ∶ 1000,the levels of IgM was higher and C3,C4 were lower than that of normal control group significantly（P 0.01）.②The serum concentrations of IgG,IgA,IgM,C3,C4 in variety of single fluorescence pattern of patients were not significantly different;And compared with every single fluorescence pattern patients,serum IgG,IgA,IgM in the bigeminy and above bigeminy fluorescence pattern patients were significantly increased,C3,C4 were significantly decreased（P 0.01）.Conclusion Although antinuclear antibody,immunoglobulin and complement highly correlated with autoimmune diseases,but not a parallel relationship with the disease.The combined detection can make a proper judgment on disease diagnosis and progression of the disease.